An evaluation of the protective effect of an infusion of chilled glucosesolution on thermal injury of the bile ducts caused by radiofrequency ablation of the liver

An evaluation of the protective effect of an infusion of chilled glucosesolution on thermal injury of the bile ducts caused by radiofrequency ablation of the liver

Author Talasso, Marcelo Salim Google Scholar
Lanzoni, Valeria Pereira Autor UNIFESP Google Scholar
Goldenberg, Alberto Autor UNIFESP Google Scholar
Garcia, Rodrigo Gobbo Google Scholar
Moura-Franco, Rita Maria Aparecida Google Scholar
Fernandes, Olívia Oléa Cisnero Google Scholar
Mesquita, Rafael de Souza Google Scholar
Carlotto, Jorge Roberto Marcante Google Scholar
Matos, Delcio Autor UNIFESP Google Scholar
Lopes Filho, Gaspar de Jesus Autor UNIFESP Google Scholar
Linhares, Marcelo Moura Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Albert Einstein Israelite Hospital Department of Image Diagnostic
Abstract PURPOSE: To evaluate the protective effects of chilling the bile ducts with cold (5°C) 5% glucose solution (GS) during radiofrequency (RF) administration. METHODS: Twenty male pigs (3 mos. old; 25-30 kg) were subjected to RF delivery with chilling (experimental group, N=10) or without chilling (control group, N=10). Half of the animals in each group were euthanized immediately after the operation, and half were euthanized one week later. The following histological variables in relation to the bile ducts were evaluated by a pathologist (blind examiner): degenerative changes to the epithelium; epithelial necrosis; ulceration, regenerative changes of the epithelium; polymorphonuclear neutrophil infiltration; and thermal effects. RESULTS: The experimental group (88 bile ducts examined) showed reduced thermal damage relative to the control group (86 bile ducts examined) as demonstrated by significant differences in the following histopathological parameters: epithelial detachment of biliary epithelium (84.1% vs. 59.3%; p<0.006); elongation/palisade arrangement of nuclei (65.1% vs. 87.5%; p<0.001); pseudo-goblet cells (32.9% vs. 56.8%; p<0.001). CONCLUSION: Infusion of 5% glucose solution (5°C) has a protective effect on bile duct subjected to heat (95-110°C, 12 min) from radiofrequency thermal ablation device.
Keywords Catheter Ablation
Radio Waves
Cold Temperature
Common Bile Duct
Liver
Intraoperative Period
Swine
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2015-01-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 30, n. 1, p. 34-45, 2015.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 34-45
Origin http://dx.doi.org/10.1590/S0102-86502015001000005
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000348793300005
SciELO ID S0102-86502015000100034 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8784

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