Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation

Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation

Author Machado, Natasha Mendonça Autor UNIFESP Google Scholar
Duailibi, Silvio Eduardo Autor UNIFESP Google Scholar
Santos, Jennifer Adriane dos Google Scholar
Penna, Vanessa Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Duailibi, Monica Talarico Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Science and Technology Institute
Abstract PURPOSE:To evaluate microscopic behavior and viability of dental pulp stem cells under glucose and glutamine deprivation.METHODS:Human tooth tissues were minced in isolated pieces and cultured until the desired cellular proliferation for experimental phases. Cells were cultured under variations of glucose and glutamine in both serum presence and absence, and then those cells were evaluated according to number and viability by MTT assay. The confocal microscopy analyzed cytoskeleton, nucleus, and mitochondria integrity.RESULTS:A low concentration of glucose favored cellular viability and microscopic behavior; the presence of glutamine in culture medium was favorable only when associated with glucose. The cellular biological potential in culture could be preserved in serum absence if nutritional requirements are adequate.CONCLUSION:Cell microscopic behavior and viability have demonstrated better patterns on serum-free low glucose culture medium with glutamine deprivation.
Keywords Dental Pulp
Culture Media
Glucose
Glutamine
Regenerative Medicine
Language English
Date 2014-10-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 10, p. 658-666, 2014.
ISSN 0102-8650 (Sherpa/Romeo)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 658-666
Origin http://dx.doi.org/10.1590/S0102-8650201400160006
Access rights Open access Open Access
Type Article
SciELO ID S0102-86502014001000658 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8627

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