Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation

Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation

Autor Machado, Natasha Mendonça Autor UNIFESP Google Scholar
Duailibi, Silvio Eduardo Autor UNIFESP Google Scholar
Santos, Jennifer Adriane dos Google Scholar
Penna, Vanessa Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Duailibi, Monica Talarico Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Science and Technology Institute
Resumo PURPOSE:To evaluate microscopic behavior and viability of dental pulp stem cells under glucose and glutamine deprivation.METHODS:Human tooth tissues were minced in isolated pieces and cultured until the desired cellular proliferation for experimental phases. Cells were cultured under variations of glucose and glutamine in both serum presence and absence, and then those cells were evaluated according to number and viability by MTT assay. The confocal microscopy analyzed cytoskeleton, nucleus, and mitochondria integrity.RESULTS:A low concentration of glucose favored cellular viability and microscopic behavior; the presence of glutamine in culture medium was favorable only when associated with glucose. The cellular biological potential in culture could be preserved in serum absence if nutritional requirements are adequate.CONCLUSION:Cell microscopic behavior and viability have demonstrated better patterns on serum-free low glucose culture medium with glutamine deprivation.
Assunto Dental Pulp
Culture Media
Glucose
Glutamine
Regenerative Medicine
Idioma Inglês
Data 2014-10-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 10, p. 658-666, 2014.
ISSN 0102-8650 (Sherpa/Romeo)
Editor Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 658-666
Fonte http://dx.doi.org/10.1590/S0102-8650201400160006
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0102-86502014001000658 (estatísticas na SciELO)
URI http://repositorio.unifesp.br/handle/11600/8627

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