Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)

Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)

Autor Carvalho, Isabel Maria Vicente Guedes De Google Scholar
Queiroz, Artur Trancoso Lopo De Google Scholar
Moraes, Rosiane Brito De Google Scholar
Gil, Helio Benites Google Scholar
Alves, Rafael Google Scholar
Viviani, Andréa De Barros Pinto Google Scholar
Becnel, James John Google Scholar
Araujo-coutinho, Carlos José Pereira Da Cunha De Google Scholar
Instituição Instituto Butantan Laboratório de Parasitologia
Fundação Oswaldo Cruz Centro de Pesquisa Gonçalo Moniz Laboratório de Imunoparasitologia
Universidade Federal de São Paulo (UNIFESP)
Superintendência de Controle de Endemias Laboratório de Entomologia Médica
Superintendência de Controle de Endemias Laboratório de Simulídeos
Mosquito and Fly Research Unit United States Department of Agriculture
Resumo Introduction Microsporidia constitute the most common black fly pathogens, although the species' diversity, seasonal occurrence and transmission mechanisms remain poorly understood. Infections by this agent are often chronic and non-lethal, but they can cause reduced fecundity and decreased longevity. The objective of this study was to identify microsporidia infecting Simulium (Chirostilbia) pertinax (Kollar, 1832) larvae from Caraguatatuba, State of São Paulo, Brazil, by molecular and morphological characterization. Methods Larvae were collected at a single point in a stream in a rural area of the city and were kept under artificial aeration until analysis. Polydispyrenia spp. infection was characterized by the presence of at least 32 mononuclear spores measuring 6.9 ± 1.0 × 5.0 ± 0.7µm in persistent sporophorous vesicles. Similarly, Amblyospora spp. were characterized by the presence of eight uninucleate spores measuring 4.5 × 3.5µm in sporophorous vesicles. Results The molecular analysis confirmed the presence of microsporidian DNA in the 8 samples (prevalence of 0.51%). Six samples (Brazilian larvae) were related to Polydispyrenia simulii and Caudospora palustris reference sequences but in separate clusters. One sample was clustered with Amblyospora spp. Edhazardia aedis was the positive control taxon. Conclusions Samples identified as Polydispyrenia spp. and Amblyospora spp. were grouped with P. simulii and Amblyospora spp., respectively, corroborating previous results. However, the 16S gene tree showed a considerable distance between the black fly-infecting Amblyospora spp. and the mosquito-infecting spp. This distance suggests that these two groups are not congeneric. Additional genomic region evaluation is necessary to obtain a coherent phylogeny for this group.
Palavra-chave Microsporidae
Amblyospora spp
Polydispyrenia spp
Phylogenetic analysis
Idioma Inglês
Data de publicação 2014-10-01
Publicado em Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 47, n. 5, p. 624-631, 2014.
ISSN 0037-8682 (Sherpa/Romeo)
Publicador Sociedade Brasileira de Medicina Tropical - SBMT
Extensão 624-631
Fonte http://dx.doi.org/10.1590/0037-8682-0165-2014
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0037-86822014000500624 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8613

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