Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions

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dc.contributor.author Bortoli, Stella
dc.contributor.author Oliveira-Silva, Diogo [UNIFESP]
dc.contributor.author Krüger, Thomas
dc.contributor.author Dörr, Felipe A.
dc.contributor.author Colepicolo, Pio
dc.contributor.author Volmer, Dietrich A.
dc.contributor.author Pinto, Ernani
dc.date.accessioned 2015-06-14T13:47:13Z
dc.date.available 2015-06-14T13:47:13Z
dc.date.issued 2014-08-01
dc.identifier http://dx.doi.org/10.1016/j.bjp.2014.07.019
dc.identifier.citation Revista Brasileira de Farmacognosia. Sociedade Brasileira de Farmacognosia, v. 24, n. 4, p. 389-398, 2014.
dc.identifier.issn 0102-695X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/8503
dc.description.abstract Cyanobacteria are prokaryotic and photosynthetic organisms, which can produce a wide range of bioactive compounds with different properties; including a variety of toxic compounds, also known as cyanotoxins. In this work, we describe the isolation of seven cyanobacterial strains from two reservoirs in São Paulo State, Brazil. Seven different chemical variants of microcystins (MC-RR, MC-LR, MC-YR, MC-LF, MC-LW, and two demethylated variants, dm-MC-RR and dm-MC-LR) were detected in three of the ten isolated strains. One particular Microcystis aeruginosa strain (LTPNA 02) was chosen to evaluate its growth by cell count, and its toxin production under seven different nutritional regimes. We observed different growth behaviors in the logarithmic growth period for only three experiments (p < 0.05). The total growth analysis identified four experiments as different from the control (p < 0.01). Three microcystin variants (MC-RR, MC-LR and MC-YR) were quantified by liquid chromatography-tandem mass spectrometry. At the experimental end, the toxin content was unchanged when comparing cell growth in ASM-1 (N:P = 1), MLA and BG-11 (N:P = 10) medium. In all other experiments, the lowest microcystin production was observed from cells grown in Bold 3N medium during the exponential growth phase. The highest microcystin content was observed in cultures using BG-11(N:P = 100) medium. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Alfried Krupp von Bohlen und Halbach-Stiftung
dc.format.extent 389-398
dc.language.iso eng
dc.publisher Sociedade Brasileira de Farmacognosia
dc.relation.ispartof Revista Brasileira de Farmacognosia
dc.rights Acesso aberto
dc.subject Cyanobacteria en
dc.subject Microcystis sp. en
dc.subject Microcystin en
dc.subject LC-MS/MS en
dc.title Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions en
dc.type Artigo
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Saarland University Institute of Bioanalytical Chemistry
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Friedrich-Schiller University of Jena Institute of Nutrition
dc.contributor.institution Hans Knöll Institute Leibniz Institute for Natural Product Research and Infection Biology
dc.description.affiliation Universidade de São Paulo Departamento de Análises Clínicas e Toxicológicas
dc.description.affiliation Saarland University Institute of Bioanalytical Chemistry
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Instituto de Ciências Ambientais, Químicas e Farmacêuticas
dc.description.affiliation Friedrich-Schiller University of Jena Institute of Nutrition
dc.description.affiliation Hans Knöll Institute Leibniz Institute for Natural Product Research and Infection Biology
dc.description.affiliation Universidade de São Paulo Instituto de Química Departamento de Bioquímica
dc.description.affiliationUnifesp UNIFESP, Instituto de Ciências Ambientais, Químicas e Farmacêuticas
dc.description.sponsorshipID CNPq: 201609/2012-6
dc.identifier.file S0102-695X2014000400389.pdf
dc.identifier.scielo S0102-695X2014000400389
dc.identifier.doi 10.1016/j.bjp.2014.07.019
dc.description.source SciELO
dc.identifier.wos WOS:000344329500004



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