Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions

Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions

Autor Bortoli, Stella Google Scholar
Oliveira-Silva, Diogo Autor UNIFESP Google Scholar
Krüger, Thomas Google Scholar
Dörr, Felipe A. Google Scholar
Colepicolo, Pio Google Scholar
Volmer, Dietrich A. Google Scholar
Pinto, Ernani Google Scholar
Instituição Universidade de São Paulo (USP)
Saarland University Institute of Bioanalytical Chemistry
Universidade Federal de São Paulo (UNIFESP)
Friedrich-Schiller University of Jena Institute of Nutrition
Hans Knöll Institute Leibniz Institute for Natural Product Research and Infection Biology
Resumo Cyanobacteria are prokaryotic and photosynthetic organisms, which can produce a wide range of bioactive compounds with different properties; including a variety of toxic compounds, also known as cyanotoxins. In this work, we describe the isolation of seven cyanobacterial strains from two reservoirs in São Paulo State, Brazil. Seven different chemical variants of microcystins (MC-RR, MC-LR, MC-YR, MC-LF, MC-LW, and two demethylated variants, dm-MC-RR and dm-MC-LR) were detected in three of the ten isolated strains. One particular Microcystis aeruginosa strain (LTPNA 02) was chosen to evaluate its growth by cell count, and its toxin production under seven different nutritional regimes. We observed different growth behaviors in the logarithmic growth period for only three experiments (p < 0.05). The total growth analysis identified four experiments as different from the control (p < 0.01). Three microcystin variants (MC-RR, MC-LR and MC-YR) were quantified by liquid chromatography-tandem mass spectrometry. At the experimental end, the toxin content was unchanged when comparing cell growth in ASM-1 (N:P = 1), MLA and BG-11 (N:P = 10) medium. In all other experiments, the lowest microcystin production was observed from cells grown in Bold 3N medium during the exponential growth phase. The highest microcystin content was observed in cultures using BG-11(N:P = 100) medium.
Palavra-chave Cyanobacteria
Microcystis sp.
Microcystin
LC-MS/MS
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Alfried Krupp von Bohlen und Halbach-Stiftung
Número do financiamento CNPq: 201609/2012-6
Data de publicação 2014-08-01
Publicado em Revista Brasileira de Farmacognosia. Sociedade Brasileira de Farmacognosia, v. 24, n. 4, p. 389-398, 2014.
ISSN 0102-695X (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira de Farmacognosia
Extensão 389-398
Fonte http://dx.doi.org/10.1016/j.bjp.2014.07.019
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000344329500004
SciELO S0102-695X2014000400389 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8503

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