Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions

Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions

Author Bortoli, Stella Google Scholar
Oliveira-Silva, Diogo Autor UNIFESP Google Scholar
Krüger, Thomas Google Scholar
Dörr, Felipe A. Google Scholar
Colepicolo, Pio Google Scholar
Volmer, Dietrich A. Google Scholar
Pinto, Ernani Google Scholar
Institution Universidade de São Paulo (USP)
Saarland University Institute of Bioanalytical Chemistry
Universidade Federal de São Paulo (UNIFESP)
Friedrich-Schiller University of Jena Institute of Nutrition
Hans Knöll Institute Leibniz Institute for Natural Product Research and Infection Biology
Abstract Cyanobacteria are prokaryotic and photosynthetic organisms, which can produce a wide range of bioactive compounds with different properties; including a variety of toxic compounds, also known as cyanotoxins. In this work, we describe the isolation of seven cyanobacterial strains from two reservoirs in São Paulo State, Brazil. Seven different chemical variants of microcystins (MC-RR, MC-LR, MC-YR, MC-LF, MC-LW, and two demethylated variants, dm-MC-RR and dm-MC-LR) were detected in three of the ten isolated strains. One particular Microcystis aeruginosa strain (LTPNA 02) was chosen to evaluate its growth by cell count, and its toxin production under seven different nutritional regimes. We observed different growth behaviors in the logarithmic growth period for only three experiments (p < 0.05). The total growth analysis identified four experiments as different from the control (p < 0.01). Three microcystin variants (MC-RR, MC-LR and MC-YR) were quantified by liquid chromatography-tandem mass spectrometry. At the experimental end, the toxin content was unchanged when comparing cell growth in ASM-1 (N:P = 1), MLA and BG-11 (N:P = 10) medium. In all other experiments, the lowest microcystin production was observed from cells grown in Bold 3N medium during the exponential growth phase. The highest microcystin content was observed in cultures using BG-11(N:P = 100) medium.
Keywords Cyanobacteria
Microcystis sp.
Microcystin
LC-MS/MS
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Alfried Krupp von Bohlen und Halbach-Stiftung
Grant number CNPq: 201609/2012-6
Date 2014-08-01
Published in Revista Brasileira de Farmacognosia. Sociedade Brasileira de Farmacognosia, v. 24, n. 4, p. 389-398, 2014.
ISSN 0102-695X (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira de Farmacognosia
Extent 389-398
Origin http://dx.doi.org/10.1016/j.bjp.2014.07.019
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000344329500004
SciELO ID S0102-695X2014000400389 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8503

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