Comparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazil

Comparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazil

Author Salustiano, Daniela Medeiros Google Scholar
Lima, Kledoaldo Oliveira de Google Scholar
Cavalcanti, Ana Maria Salustiano Google Scholar
Diaz, Ricardo Sobhie Autor UNIFESP Google Scholar
Lacerda, Heloisa Ramos Google Scholar
Institution The Herbert de Souza Counselling and Testing Centre
Universidade Federal de Pernambuco
The Paulista Counselling and Testing Centre
Laboratório Central de Pernambuco Department of Virology
Universidade Federal de São Paulo (UNIFESP)
Abstract The aims of this study were to compare the automated AxSYM avidity assay index with the BED capture enzyme immunoassay test and to calculate the HIV-1 incidence using the BED capture enzyme immunoassay and AxSYM avidity assay index algorithms within a population seeking the Voluntary Counselling and Testing Centres in two municipalities in the Metropolitan Region of Recife, Northeast of Brazil. An analysis was conducted in 365 samples that tested positive for HIV infection from frozen serum collected during the period 2006-2009. There was a similar proportion of males and females; most patients were heterosexual (86%) with a median age of 29 years. Of the 365 samples, 102 (28%) and 66 (18.1%) were identified as recent infections by BED capture enzyme immunoassay and AxSYM avidity assay index, respectively. The HIV-1 total incidence in the BED capture enzyme immunoassay and AxSYM avidity assay index algorithms were: 0.79 (95% CI: 0.60-0.98) and 0.34 (95% CI: -0.04 to 0.72), respectively. Incidence was higher among men. There was good agreement between the tests, with a kappa of 0.654 and a specificity of 95.8%. AxSYM avidity assay index may be helpful in improving the quality of the estimates of recent HIV infection and incidence, particularly when used in a combined algorithm with BED capture enzyme immunoassay.
Keywords HIV
Recent infection
Avidity index assay
BED-CEIA
Language English
Sponsor Programa Nacional de Cooperação Acadêmica
Ação Novas Fronteiras (Procad - NF)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2014-07-01
Published in Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 18, n. 4, p. 449-453, 2014.
ISSN 1413-8670 (Sherpa/Romeo, impact factor)
Publisher Brazilian Society of Infectious Diseases
Extent 449-453
Origin http://dx.doi.org/10.1016/j.bjid.2014.03.001
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000341171200015
SciELO ID S1413-86702014000400449 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8492

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