Immunohistochemical evaluation of proliferation, apoptosis and steroidogenic enzymes in the ovary of rats with polycystic ovary

Show simple item record

dc.contributor.author Lombardi, Leonardo Augusto
dc.contributor.author Simões, Ricardo Santos
dc.contributor.author Maganhin, Carla Cristina [UNIFESP]
dc.contributor.author Baracat, Maria Cândida Pinheiro
dc.contributor.author Silva-Sasso, Gisela Rodrigues
dc.contributor.author Florencio-Silva, Rinaldo [UNIFESP]
dc.contributor.author Soares Júnior, José Maria [UNIFESP]
dc.contributor.author Baracat, Edmund Chada [UNIFESP]
dc.date.accessioned 2015-06-14T13:47:11Z
dc.date.available 2015-06-14T13:47:11Z
dc.date.issued 2014-07-01
dc.identifier http://dx.doi.org/10.1590/1806-9282.60.04.0014
dc.identifier.citation Revista da Associação Médica Brasileira. Associação Médica Brasileira, v. 60, n. 4, p. 349-356, 2014.
dc.identifier.issn 0104-4230
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/8472
dc.description.abstract Objective:to evaluate the immunohistochemical expression of proliferative, apoptotic and steroidogenic enzyme markers in the ovaries of rats with polycystic ovary syndrome (PCOS).Methods:twenty rats were divided into two groups: GCtrl - estrous phase, and PCOS - with polycystic ovaries. The GCtrl animals were subjected to a lighting period from 7 am to 7 pm, while the animals with PCOS group remained with continuous lighting for 60 days. Subsequently, the animals were anesthetized, the ovaries were removed and fixed in 10% formaldehyde, prior to paraffin embedding. Sections were stained using H.E. or subjected to immunohistochemical methods for the detection of Ki-67, cleaved caspase-3, CYP11A1, CYP17A1 and CYP19A1. The results were analyzed using Student's t-test (p < 0,05).Results:morphological results showed evidence of interstitial cells originating from the inner theca cells of degenerating ovarian cysts in PCOS. Immunoexpression of Ki-67 was higher in the granulosa cells in GCtrl, and the theca interna cells in PCOS, while cleaved caspase-3 was higher in granulosa cells of ovarian cysts from PCOS and in the theca interna cells of GCtrl. Immunoreactivity of CYP11A1 in the theca interna, granulosa and interstitial cells was similar between the two groups, while CYP17A1 and CYP19A1 were higher in the granulosa and interstitial cells in the PCOS group.Conclusion:the results indicate that the interstitial cells are derived from the theca interna and that enzymatic changes occur in the theca interna and interstitial cells in ovaries of rats with PCOS, responsible for the high levels of androgens and estradiol. en
dc.description.abstract Objetivo:avaliar a expressão imunoistoquímica de marcadores de proliferação, apoptose e enzimas esteroidogênicas nos ovários de ratas com síndrome dos ovários policísticos (SOP).Métodos:vinte ratas foram divididas em dois grupos: controle (GCtrl), na fase de estro, e com síndrome dos ovários policísticos (GSOP). Os animais do GCtrl permaneceram com período de luz das 7 às 19 horas, e os do GSOP com iluminação contínua, durante 60 dias. Posteriormente, os animais foram anestesiados, os ovários removidos e fixados em formol a 10% para inclusão em parafina. Cortes histológicos foram corados pelo H.E. e outros submetidos a métodos imunoistoquímicos para detecção de Ki-67, caspase 3 clivada, CYP11A1, CYP17A1 e CYP19A1. Os resultados foram submetidos ao teste t de Student (p < 0,05).Resultados:a morfologia mostrou evidências da origem das células intersticiais a partir das células da teca interna dos cistos ovarianos em degeneração no GSOP. A imunoexpressão do Ki-67 mostrou-se aumentada nas células da granulosa no GCtrl e na teca interna do GSOP, enquanto a caspase 3 clivada se mostrou aumentada nas células da granulosa dos cistos ovarianos do GSOP e na teca interna do GCtrl. A imunorreatividade da CYP11A1 nas células da teca interna, bem como da granulosa e intersticiais, mostrou-se semelhante entre os dois grupos. As CYP17A1 e CYP19A1 apresentaram-se aumentadas nas células da granulosa e intersticiais no grupo SOP.Conclusão:os resultados indicam que as células intersticiais são oriundas da teca interna e que ocorrem alterações enzimáticas nas células da teca interna e intersticiais do ovário de ratas com SOP, responsáveis pelos altos níveis de androgênios e de estradiol. pt
dc.format.extent 349-356
dc.language.iso eng
dc.publisher Associação Médica Brasileira
dc.relation.ispartof Revista da Associação Médica Brasileira
dc.rights Acesso aberto
dc.subject polycystic ovary syndrome en
dc.subject ovary en
dc.subject ovarian cysts en
dc.subject apoptosis en
dc.subject cell proliferation en
dc.subject cytochrome P-450 enzyme eystem en
dc.subject rats en
dc.subject female en
dc.subject síndrome do ovário policístico pt
dc.subject ovário pt
dc.subject cistos ovarianos pt
dc.subject apoptose pt
dc.subject proliferação celular pt
dc.subject sistema enzimático do citocromo P-450 pt
dc.subject ratos pt
dc.subject feminino pt
dc.title Immunohistochemical evaluation of proliferation, apoptosis and steroidogenic enzymes in the ovary of rats with polycystic ovary en
dc.title.alternative Avaliação imunoistoquímica de proliferação, apoptose e enzimas esteroidogênicas no ovário de ratas com síndrome dos ovários policísticos pt
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution University of São Paulo Medical School Obstetrics and Gynecology Department
dc.description.affiliation Federal University of São Paulo Paulista Medical School Morphology and Genetics Department
dc.description.affiliation University of São Paulo Medical School Obstetrics and Gynecology Department
dc.description.affiliationUnifesp UNIFESP, EPM, Morphology and Genetics Department
dc.identifier.file S0104-42302014000400349.pdf
dc.identifier.scielo S0104-42302014000400349
dc.identifier.doi 10.1590/1806-9282.60.04.0014
dc.description.source SciELO



File

Name: S0104-42302014000400349.pdf
Size: 922.0Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account