Effects of maternal ischemic preconditioning in the colon of newborn rats submitted to hypoxia-reoxygenation insult

Effects of maternal ischemic preconditioning in the colon of newborn rats submitted to hypoxia-reoxygenation insult

Autor Freitas, Maria Andréia Lopes de Google Scholar
Gomes, Rúdnei de Oliveira Luciano Autor UNIFESP Google Scholar
Soares, Bruno Leonardo de Freitas Google Scholar
Artigiani Neto, Ricardo Autor UNIFESP Google Scholar
Montero, Edna Frasson de Souza Autor UNIFESP Google Scholar
Martins, Jose Luiz Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Faculty of Medicine of Marilia
UNIVASF Faculty of Medicine
Universidade de São Paulo (USP)
Resumo PURPOSE:To evaluate the effects of maternal remote ischemic preconditioning (IPCr) in the colonic mucosa of newborn rats subjected to hypoxia and reoxygenation.METHODS: Newborn Wistar rats were divided into three groups. Control Group (CG), Hypoxia and Reoxygenation Group (HRG) and Remote Ischemic Preconditioning Group (IPCrG). Hypoxia and reoxygenation was performed 2x per day, with an interval of 6 hours, on the 1st, 2nd and 3rd days of life, with 10 minutes of CO2 at 100%, followed by 10 minutes O2 at 100%(HRG/IPCrG). The maternal IPCr was performed 24 hours before delivery by applying a rubber band tourniquet to the left hind limb (IPCrG). Segments of the colon underwent histological (HE) and immunohistochemical analysis for caspase-3 and COX - 2.RESULTS:The histological findings showed no intestinal mucosal damage in the CG group and severe lesions in HRG that was attenuated in the IPCrG (p<0.05). The expression of the apoptotic cells was lower in the HRG group than in the CG and IPCrG. The COX-2 expression was intense in HRG and attenuated in the IPCrG (p<0.05).CONCLUSIONS:Maternal IPCr protected the colonic mucosa of newborn rats subjected to hypoxia and reoxygenation, reducing the morphological alterations and inflammatory response. It ameliorates the occurrence of apoptosis, keeping the physiological process of renewal and regeneration in the epithelial lining of the colonic mucosa.
Palavra-chave Enterocolitis
Necrotizing
Colon
Apoptosis
Rats
Idioma Inglês
Data de publicação 2014-07-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 7, p. 438-444, 2014.
ISSN 0102-8650 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 438-444
Fonte http://dx.doi.org/10.1590/S0102-86502014000700005
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000340835400005
SciELO S0102-86502014000700438 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8467

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