Effect of a submaxillary gland extract on Ehrlich tumor growth in mice

Effect of a submaxillary gland extract on Ehrlich tumor growth in mice

Autor Weill, P. Google Scholar
Frussa-Filho, Roberto Autor UNIFESP Google Scholar
Bonamin, Leoni Villano Google Scholar
Instituição Universidade Paulista
Universidade Federal de São Paulo (UNIFESP)
Resumo Ablation of host submaxillary glands modifies Ehrlich tumor growth and tumor-infiltrating leukocytes, possibly by modifications in the serum level of growth factors produced by this gland. To extend this research, 7-month-old male EPM-1 mice (N = 30) were divided into two groups: 1) inoculated with tumor cells previously incubated with submaxillary salivary gland extract (SGE) in PBS for 30 min at 37%; 2) inoculated with tumor cells previously incubated with PBS, under the same conditions. Animals were inoculated into the footpad with 40 µl of a suspension containing 4.5 x 107 tumor cells/ml, and footpad thickness was measured daily for 10 days. Sections and smears of tumor cells were prepared from the tumor mass to determine mitosis frequency, percent of tumor cells immunopositive to nerve (NGF) and epidermal (EGF) growth factors and percent of tumor-infiltrating leukocytes. The incubation of tumor cells with SGE produced a tumor reduction of about 30% in size (P<0.01). This effect was not related to loss of cell viability during incubation, but a 33% increase (P<0.05) in the percentage of dead or dying tumor cells and a 15% increase in the percent of NGF/EGF-positive tumor cells (P<0.01) were observed in vivo at the end of experiment. Tumor-infiltrating lymphocytes and mitosis frequency did not differ between groups. These data suggest a direct effect of factors present in SGE on tumor cells, which induce degeneration of tumor cells.
Palavra-chave Ehrlich ascites carcinoma
submaxillary salivary gland
growth factors
Idioma Inglês
Data de publicação 1999-10-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 10, p. 1205-1209, 1999.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 1205-1209
Fonte http://dx.doi.org/10.1590/S0100-879X1999001000005
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000083389700005
SciELO S0100-879X1999001000005 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/846

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