Evaluation of Toll-like, chemokine, and integrin receptors on monocytes and neutrophils from peripheral blood of septic patients and their correlation with clinical outcomes

Evaluation of Toll-like, chemokine, and integrin receptors on monocytes and neutrophils from peripheral blood of septic patients and their correlation with clinical outcomes

Autor Silva, Selma Cristina da Autor UNIFESP Google Scholar
Baggio-Zappia, G.l. Google Scholar
Brunialti, Milena Karina Coló Autor UNIFESP Google Scholar
Assunçao, M.s.c. Google Scholar
Azevedo, Luciano Cesar Pontes Autor UNIFESP Google Scholar
Machado, Flávia Ribeiro Autor UNIFESP Google Scholar
Salomão, Reinaldo Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Hospital Israelita Albert Einstein Unidade de Terapia Intensiva
Hospital Sírio Libanês Unidade de Terapia Intensiva
Resumo Recognition of pathogens is performed by specific receptors in cells of the innate immune system, which may undergo modulation during the continuum of clinical manifestations of sepsis. Monocytes and neutrophils play a key role in host defense by sensing and destroying microorganisms. This study aimed to evaluate the expression of CD14 receptors on monocytes; CD66b and CXCR2 receptors on neutrophils; and TLR2, TLR4, TLR5, TLR9, and CD11b receptors on both cell types of septic patients. Seventy-seven septic patients (SP) and 40 healthy volunteers (HV) were included in the study, and blood samples were collected on day zero (D0) and after 7 days of therapy (D7). Evaluation of the cellular receptors was carried out by flow cytometry. Expression of CD14 on monocytes and of CD11b and CXCR2 on neutrophils from SP was lower than that from HV. Conversely, expression of TLR5 on monocytes and neutrophils was higher in SP compared with HV. Expression of TLR2 on the surface of neutrophils and that of TLR5 on monocytes and neutrophils of SP was lower at D7 than at D0. In addition, SP who survived showed reduced expression of TLR2 and TLR4 on the surface of neutrophils at D7 compared to D0. Expression of CXCR2 for surviving patients was higher at follow-up compared to baseline. We conclude that expression of recognition and cell signaling receptors is differentially regulated between SP and HV depending on the receptor being evaluated.
Palavra-chave Sepsis
Toll-like receptors
TLR5
TLR9
CXCR2
Idioma Inglês
Data de publicação 2014-05-02
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 47, n. 5, p. 384-393, 2014.
ISSN 0100-879X (Sherpa/Romeo)
Publicador Associação Brasileira de Divulgação Científica
Extensão 384-393
Fonte http://dx.doi.org/10.1590/1414-431X20143190
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0100-879X2014000500384 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8379

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