Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin

Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin

Autor Raymundo, Erica Calcagno Google Scholar
Hochman, Bernardo Autor UNIFESP Google Scholar
Nishioka, Michele Akemi Autor UNIFESP Google Scholar
Freitas, Jose Octavio Gonçalves de Autor UNIFESP Google Scholar
Maximino, Jéssica Ruivo Google Scholar
Chadi, Gerson Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo PURPOSE:To investigate the subcutaneous injection of carbon dioxide (CO2) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin. METHODS:Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO2 injection (CO2Inj) and atmospheric air injection (AirInj) - with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB). RESULTS:In SP group, there was a decrease in the amount of neuropeptides in subgroups CO2Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO2Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups. CONCLUSION:Subcutaneous injection of CO2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin Gene-Related Peptide (15 kDa) neuropeptides in rat skin.
Palavra-chave Carbon Dioxide
Substance P
Calcitonin Gene-Related Peptide
Neurogenic Inflammation
Skin
Rats
Idioma Inglês
Data de publicação 2014-04-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 4, p. 224-230, 2014.
ISSN 0102-8650 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 224-230
Fonte http://dx.doi.org/10.1590/S0102-86502014000400002
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000334997000002
SciELO S0102-86502014000400224 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8346

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