Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in rats

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dc.contributor.author Perez, Rogerio Renato
dc.contributor.author Goldenberg, Alberto [UNIFESP]
dc.contributor.author Netto, Alcides Augusto Salzedas
dc.contributor.author Gonzalez, Adriano Miziara [UNIFESP]
dc.date.accessioned 2015-06-14T13:46:59Z
dc.date.available 2015-06-14T13:46:59Z
dc.date.issued 2014-03-01
dc.identifier http://dx.doi.org/10.1590/S0102-86502014000300005
dc.identifier.citation Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 3, p. 171-177, 2014.
dc.identifier.issn 0102-8650
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/8286
dc.description.abstract PURPOSE:To compare the efficacy of different types of solutions (Belzer or Euro-Collins) for the preservation of rat pancreas during cold ischemia. METHODS:Thirty Wistar rats were divided into three groups according to the perfusion or storage solution: Group E (perfusion and storage in Euro-Collins solution); Group B (perfusion and storage in Belzer solution) and Group BE (Perfusion in Belzer solution and storage in Euro-Collins solution). After perfusion, the pancreas was excised and stored at 4˚C for 18 hours. Amylase was measured at 6, 12 and 18h, and histological analysis of the pancreas was performed after 18h of cold storage.RESULTS:Amylase was elevated and comparable in Groups E and BE after 12 and 18 hours of ischemia (p<0.05). In the exocrine pancreas, histological differences in the amount of necrosis (p=0.049), lymphocytic infiltrate (p<0.001) and neutrophilic infiltrate (p=0.004) were observed, with more favorable features present in Group B. In the endocrine pancreas, Group B showed less edema (p<0.001), but other parameters were similar among all groups. CONCLUSION:The Euro-Collins solution is inferior to the Belzer solution for the preservation of rat pancreas during cold ischemia. en
dc.format.extent 171-177
dc.language.iso eng
dc.publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartof Acta Cirurgica Brasileira
dc.rights Acesso aberto
dc.subject Diabetes Mellitus, Type 1 en
dc.subject Islets of Langerhans Transplantation en
dc.subject Organ Preservation Solutions en
dc.subject Rats en
dc.title Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in rats en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation São Paulo Federal University
dc.description.affiliation UNIFESP Paulista School of Medicine Department of Surgery
dc.description.affiliation UNIFESP-EPM Department of Surgery
dc.description.affiliationUnifesp São Paulo Federal University
dc.description.affiliationUnifesp UNIFESP, Paulista School of Medicine Department of Surgery
dc.description.affiliationUnifesp UNIFESP, EPM Department of Surgery
dc.identifier.file S0102-86502014000300171.pdf
dc.identifier.scielo S0102-86502014000300171
dc.identifier.doi 10.1590/S0102-86502014000300005
dc.description.source SciELO
dc.identifier.wos WOS:000333047100005



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