Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in rats

Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in rats

Autor Perez, Rogerio Renato Google Scholar
Goldenberg, Alberto Autor UNIFESP Google Scholar
Netto, Alcides Augusto Salzedas Google Scholar
Gonzalez, Adriano Miziara Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo PURPOSE:To compare the efficacy of different types of solutions (Belzer or Euro-Collins) for the preservation of rat pancreas during cold ischemia. METHODS:Thirty Wistar rats were divided into three groups according to the perfusion or storage solution: Group E (perfusion and storage in Euro-Collins solution); Group B (perfusion and storage in Belzer solution) and Group BE (Perfusion in Belzer solution and storage in Euro-Collins solution). After perfusion, the pancreas was excised and stored at 4˚C for 18 hours. Amylase was measured at 6, 12 and 18h, and histological analysis of the pancreas was performed after 18h of cold storage.RESULTS:Amylase was elevated and comparable in Groups E and BE after 12 and 18 hours of ischemia (p<0.05). In the exocrine pancreas, histological differences in the amount of necrosis (p=0.049), lymphocytic infiltrate (p<0.001) and neutrophilic infiltrate (p=0.004) were observed, with more favorable features present in Group B. In the endocrine pancreas, Group B showed less edema (p<0.001), but other parameters were similar among all groups. CONCLUSION:The Euro-Collins solution is inferior to the Belzer solution for the preservation of rat pancreas during cold ischemia.
Palavra-chave Diabetes Mellitus, Type 1
Islets of Langerhans Transplantation
Organ Preservation Solutions
Rats
Idioma Inglês
Data de publicação 2014-03-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 3, p. 171-177, 2014.
ISSN 0102-8650 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 171-177
Fonte http://dx.doi.org/10.1590/S0102-86502014000300005
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000333047100005
SciELO S0102-86502014000300171 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8286

Exibir registro completo




Arquivo

Nome: S0102-86502014000300171.pdf
Tamanho: 1.780MB
Formato: PDF
Descrição:
Abrir arquivo

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta