Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury

Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury

Autor Ikejiri, Adauto Tsutomu Google Scholar
Somaio Neto, Frederico Google Scholar
Chaves, Jose Carlos Google Scholar
Bertoletto, Paulo Roberto Google Scholar
Teruya, Roberto Google Scholar
Bertoletto, Eduardo Rodrigues Google Scholar
Taha, Murched Omar Autor UNIFESP Google Scholar
Fagundes, Djalma José Autor UNIFESP Google Scholar
Instituição Federal University of Grande Dourados Medical School
UFGD Medical School
Federal University of Mato Grosso do Sul Surgery Department
Universitary Center Sao Camilo
Universidade Federal de São Paulo (UNIFESP)
Resumo PURPOSE:To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion.METHODS:Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm [2
Palavra-chave Gene Expression
Oxidative Stress
Lung
Reperfusion Injury
Mice
Idioma Inglês
Data de publicação 2014-03-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 3, p. 186-192, 2014.
ISSN 0102-8650 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 186-192
Fonte http://www.scielo.br/scielo.php?pid=S0102-86502014000300186&script=sci_arttext&tlng=pt
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000333047100007
SciELO S0102-86502014000300186 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8265

Exibir registro completo




Arquivo

Nome: S0102-86502014000300186.pdf
Tamanho: 349.9KB
Formato: PDF
Descrição:
Abrir arquivo

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta