Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats

Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats

Author Suartz, Caio Vinicius Autor UNIFESP Google Scholar
Gaiba, Silvana Google Scholar
França, Jerônimo Pereira de Google Scholar
Aloise, Antonio Carlos Autor UNIFESP Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Santa Cruz Department of Biological Sciences
Abstract PURPOSE:To evaluate the effects of the adipose-derived stem cells (ADSC) in the viability of random skin flap in rats.METHODS:Thirty five adult male Wistar rats (weight 250-300 g) were used. ADSC were isolated from adult male rats (n=5). ADSC were separated, cultured and then analyzed. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surgical procedure, the animals were randomized into two groups (n=15 each group), group control and group ADSC. In all groups the procedures were performed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS:The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSION: The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random skin flaps in rats.
Keywords Surgical Flaps
Rats
Adult Stem Cells
Stem Cells
Adipose Tissue
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number CNPq: 312356/2009-9
Date 2014-01-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 6-9, 2014.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 6-9
Origin http://dx.doi.org/10.1590/S0102-86502014001400002
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000342065300003
SciELO ID S0102-86502014001400003 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8209

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