Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats

Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats

Autor Suartz, Caio Vinicius Google Scholar
Gaiba, Silvana Google Scholar
França, Jerônimo Pereira de Google Scholar
Aloise, Antonio Carlos Autor UNIFESP Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Santa Cruz Department of Surgery
Resumo PURPOSE:To evaluate the viability of random pattern dorsal skin flaps in rats after injection of adipose-derived stem cells (ADSC).METHODS: Thirty five adult male Wistar EPM rats (weight 250-300 g) were distributed, at random, in two groups. I- Control (flap elevation with injection of saline solution) with fifteen animals and II- Experimental (flap elevation with injection of ADSC ) with fifteen animal. The ADSC were isolated from others five adult male rats. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed in both groups and the injection (cells or saline solution) were perfomed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS:The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation to verify their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSIONS:The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random random pattern dorsal skin flaps in rats.
Palavra-chave Surgical Flaps
Rats
Adult Stem Cells
Stem Cells
Adipose Tissue
Idioma Inglês
Financiador Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Número do financiamento CNPq: 312356/2009-9
Data de publicação 2014-01-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 02-05, 2014.
ISSN 0102-8650 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 02-05
Fonte http://dx.doi.org/10.1590/S0102-86502014001700001
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000344466300002
SciELO S0102-86502014001500002 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8208

Exibir registro completo




Arquivo

Nome: S0102-86502014001500002.pdf
Tamanho: 400.8KB
Formato: PDF
Descrição:
Abrir arquivo

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta