Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats

Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats

Author Suartz, Caio Vinicius Google Scholar
Gaiba, Silvana Google Scholar
França, Jerônimo Pereira de Google Scholar
Aloise, Antonio Carlos Autor UNIFESP Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Santa Cruz Department of Surgery
Abstract PURPOSE:To evaluate the viability of random pattern dorsal skin flaps in rats after injection of adipose-derived stem cells (ADSC).METHODS: Thirty five adult male Wistar EPM rats (weight 250-300 g) were distributed, at random, in two groups. I- Control (flap elevation with injection of saline solution) with fifteen animals and II- Experimental (flap elevation with injection of ADSC ) with fifteen animal. The ADSC were isolated from others five adult male rats. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed in both groups and the injection (cells or saline solution) were perfomed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS:The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation to verify their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSIONS:The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random random pattern dorsal skin flaps in rats.
Keywords Surgical Flaps
Rats
Adult Stem Cells
Stem Cells
Adipose Tissue
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number CNPq: 312356/2009-9
Date 2014-01-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 02-05, 2014.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 02-05
Origin http://dx.doi.org/10.1590/S0102-86502014001700001
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000344466300002
SciELO ID S0102-86502014001500002 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8208

Show full item record




File

Name: S0102-86502014001500002.pdf
Size: 400.8Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account