Alprostadil attenuates inflammatory aspects and leucocytes adhesion on renal ischemia and reperfusion injury in rats

Alprostadil attenuates inflammatory aspects and leucocytes adhesion on renal ischemia and reperfusion injury in rats

Author Soares, Bruno Leonardo de Freitas Autor UNIFESP Google Scholar
Freitas, Maria Andréia Lopes de Google Scholar
Montero, Edna Frasson de Souza Autor UNIFESP Google Scholar
Pitta, Guilherme Benjamin Brandão Google Scholar
Miranda Júnior, Fausto Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Federal University do Vale do São Francisco Faculty of Nursing
University of São Paulo Faculty of Medicine Department of Surgery
University of Health Sciences of Alagoas Faculty of Medicine
Abstract PURPOSE:To evaluate the effects of alprostadil in an experimental model of ischemia and reperfusion injury (IRI) in rat renal tissue.METHODS: Adult male Wistar rats were randomized into three groups Vehicle-treated group(Veh), Alprostadil-treated(Al), and sham(Sh) group. Veh and Al groups had suprarenal aorta occluded for 30 minutes and reperfused for 60 minutes. Saline or 20 µg/kg of Alprostadil was intravenously infused immediately before declamping. Sh group animals underwent similar procedure without aortic occlusion. Left nephrectomy and blood sampling were performed after 60 minutes of reperfusion. Renal ICAM-1 expression and histological analysis were performed to estimate inflammatory response and tissue disarrangement. Serum biochemical markers for IRI were also measured. Kruskal-Wallis test was used to assess differences between the groups.RESULTS: There was lower expression of ICAM-1 in groups Veh and Sh. On histologically evaluation, inflammation and necrosis in the Veh group was significantly higher (grades III/IV) than Al group (Veh>Al=Sh; p = 0.025), as well as CPK levels (Veh>Al=Sh; p = 0.03).CONCLUSION: Alprostadil attenuates the immunohistochemical and histological repercussions in the renal tissue of rats submitted to a post-ischemic reperfusion with supra-renal aortic clamping.
Keywords ischemic preconditioning
Kidney
Oxidative stress
Cytokines
ICAM-1
Alprostadil
Language English
Date 2014-01-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 55-60, 2014.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 55-60
Origin http://dx.doi.org/10.1590/S0102-8650201400140011
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000342065300012
SciELO ID S0102-86502014001400012 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8205

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