Human beta defensin-4 and keratinocyte growth factor gene expression in cultured keratinocyte and fibroblasts of burned patients

Human beta defensin-4 and keratinocyte growth factor gene expression in cultured keratinocyte and fibroblasts of burned patients

Autor Corrêa, Silvana Aparecida Alves Autor UNIFESP Google Scholar
Noronha, Samuel Marcos Ribeiro de Google Scholar
Lanziani, Larissa Elias Google Scholar
Ipolito, Michele Zampieri Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Gragnani, Alfredo Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo PURPOSE: To evaluate KGF and human beta defensin-4 (HBD-4) levels produced by dermic fibroblasts and keratinocytes cultivated from burned patients' skin samples.METHODS: Keratinocytes and fibroblasts of 10 patients (four major burns, four minor burns and two controls) were primarily cultivated according to standard methods. HBD-4 and KGF genes were analyzed by quantitative PCR.RESULTS:In fibroblasts, KGF gene expression was 220±80 and 33.33±6.67 (M±SD; N=4), respectively for major and minor burn groups. In keratinocytes, KGF gene expression was 11.2±1.9 and 3.45±0.37 (M±SD; N=4), respectively for major and minor burn groups. In fibroblasts, HBD-4 gene expression was 15.0±4.0 and 11.5±0.5 (M±SD; N=4), respectively for major and minor burn. In keratinocyte, HBD-4 gene expression was 0.0±0.0 and 13.4±4.8 (M±SD; N=4), respectively for major and minor burn.CONCLUSIONS:KGF expression was increased in burn patient fibroblasts compared to control group. In keratinocytes culture, KGF suppression is inversely proportional to burn extension; it is active and increased in major burn but decreased in minor burn. HBD-4 expression was increased in fibroblasts and decreased in keratinocytes from all burned patients.
Palavra-chave Burns
Gene expression
Fibroblasts
Keratinocytes
Fibroblast growth factor 7
Human beta defensin 4
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Número do financiamento FAPESP: 2011/12.945-4
FAPESP: 2013/10.905-0
Data de publicação 2014-01-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 39-43, 2014.
ISSN 0102-8650 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 39-43
Fonte http://dx.doi.org/10.1590/S0102-86502014001700008
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000344466300009
SciELO S0102-86502014001500039 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8180

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