Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patients

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dc.contributor.author Noronha, Samuel Marcos Ribeiro de [UNIFESP]
dc.contributor.author Corrêa, Silvana Aparecida Alves [UNIFESP]
dc.contributor.author Klepp, Anthony Gueratto
dc.contributor.author Ipolito, Michele Zampieri
dc.contributor.author Ferreira, Lydia Masako [UNIFESP]
dc.contributor.author Gragnani, Alfredo [UNIFESP]
dc.date.accessioned 2015-06-14T13:46:52Z
dc.date.available 2015-06-14T13:46:52Z
dc.date.issued 2014-01-01
dc.identifier http://dx.doi.org/10.1590/S0102-86502014001300012
dc.identifier.citation Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 62-68, 2014.
dc.identifier.issn 0102-8650
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/8178
dc.description.abstract PURPOSE:To evaluate the level of cytokines and keratinocyte growth factor (KGF) or Fibroblast Growth Factor 7 (FGF-7) in the culture medium of cultured human dermal fibroblasts from patients with large burn in comparison to small burn.METHODS:Fibroblasts of 10 patients (four large burns, four small burns and two controls) were initiated by the enzymatic method using collagenase. Cytokines and KGF in the supernatant of the culture medium was measured by, respectively, flow cytometry using Cytometric Bead Array Human Inflammation kit (CBA, BD Biosciences, USA) and the enzyme immunoassay method using the Quantikine (r) Human KGF. The experiments were performed in triplicate.RESULTS:The expression of IL-12 protein in patients with large burns showed a tendency to increase. IL- 6, IL- 10, and IL- 1beta were observed no difference. For IL - 8, TNF - alpha and KGF was observed a significant difference between the expression in large and small burned patient.CONCLUSION:That IL-8, TNF-alpha and KGF showed higher expression in cultured fibroblasts of large burned patients. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 62-68
dc.language.iso eng
dc.publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartof Acta Cirurgica Brasileira
dc.rights Acesso aberto
dc.subject Burns en
dc.subject Fibroblasts en
dc.subject Flow Cytometry en
dc.subject Immunoenzyme Technique en
dc.subject Fibroblast Growth Factor 7 en
dc.subject Interleukins en
dc.subject Tumor Necrosis Factor Alpha en
dc.title Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patients en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation UNIFESP-EPM Department of Surgery
dc.description.affiliation UNIFESP-EPM
dc.description.affiliation UNIFESP-EPM Department of Gynecology
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP), Escola Paulista de Medicina (EPM) Department of Surgery
dc.description.affiliationUnifesp UNIFESP, EPM, Department of Surgery
dc.description.affiliationUnifesp UNIFESP-EPM
dc.description.affiliationUnifesp UNIFESP, EPM Department of Gynecology
dc.description.affiliationUnifesp UNIFESP, EPM, Department of Surgery
dc.description.sponsorshipID FAPESP: 2011/12945-4
dc.description.sponsorshipID FAPESP: 2011/23.985-7
dc.identifier.file S0102-86502014001300013.pdf
dc.identifier.scielo S0102-86502014001300013
dc.identifier.doi 10.1590/S0102-86502014001300012
dc.description.source SciELO
dc.identifier.wos WOS:000342065000013



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