Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patients

Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patients

Author Noronha, Samuel Marcos Ribeiro de Autor UNIFESP Google Scholar
Corrêa, Silvana Aparecida Alves Autor UNIFESP Google Scholar
Klepp, Anthony Gueratto Google Scholar
Ipolito, Michele Zampieri Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Gragnani, Alfredo Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE:To evaluate the level of cytokines and keratinocyte growth factor (KGF) or Fibroblast Growth Factor 7 (FGF-7) in the culture medium of cultured human dermal fibroblasts from patients with large burn in comparison to small burn.METHODS:Fibroblasts of 10 patients (four large burns, four small burns and two controls) were initiated by the enzymatic method using collagenase. Cytokines and KGF in the supernatant of the culture medium was measured by, respectively, flow cytometry using Cytometric Bead Array Human Inflammation kit (CBA, BD Biosciences, USA) and the enzyme immunoassay method using the Quantikine (r) Human KGF. The experiments were performed in triplicate.RESULTS:The expression of IL-12 protein in patients with large burns showed a tendency to increase. IL- 6, IL- 10, and IL- 1beta were observed no difference. For IL - 8, TNF - alpha and KGF was observed a significant difference between the expression in large and small burned patient.CONCLUSION:That IL-8, TNF-alpha and KGF showed higher expression in cultured fibroblasts of large burned patients.
Keywords Burns
Fibroblasts
Flow Cytometry
Immunoenzyme Technique
Fibroblast Growth Factor 7
Interleukins
Tumor Necrosis Factor Alpha
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2011/12945-4
FAPESP: 2011/23.985-7
Date 2014-01-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 62-68, 2014.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 62-68
Origin http://dx.doi.org/10.1590/S0102-86502014001300012
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000342065000013
SciELO ID S0102-86502014001300013 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8178

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