Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

Author Lucon, Marcos Google Scholar
Martins, Joao Roberto Google Scholar
Leite, Kátia Ramos Moreira Autor UNIFESP Google Scholar
Soler, Roberto Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Srougi, Miguel Autor UNIFESP Google Scholar
Bruschini, Homero Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Introduction: Painful bladder syndrome/interstitial cystitis (PBS/IC) pathogenesis is not fully known, but evidence shows that glycosaminoglycans (GAG) of bladder urothelium can participate in its genesis. The loss of these compounds facilitates the contact of urine compounds with deeper portions of bladder wall triggering an inflammatory process. We investigated GAG in urine and tissue of PBS/IC and pure stress urinary incontinence (SUI) patients to better understand its metabolism. Materials and Methods: Tissue and urine of 11 patients with PBS/IC according to NIDDK criteria were compared to 11 SUI patients. Tissue samples were analyzed by histological, immunohistochemistry and immunofluorescence methods. Statistical analysis were performed using t Student test and Anova, considering significant when p < 0.05. Results: PBS/IC patients had lower concentration of GAG in urine when compared to SUI (respectively 0.45 ± 0.11 x 0.62 ± 0.13 mg/mg creatinine, p < 0.05). However, there was no reduction of the content of GAG in the urothelium of both groups. Immunofluorescence showed that PBS/IC patients had a stronger staining of TGF-beta, decorin (a proteoglycan of chondroitin/dermatan sulfate), fibronectin and hyaluronic acid. Conclusion: the results suggest that GAG may be related to the ongoing process of inflammation and remodeling of the dysfunctional urothelium that is present in the PBS/IC.
Keywords Cystitis, Interstitial
Glycosaminoglycans
Hyaluronic Acid
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2014-01-01
Published in International braz j urol. Sociedade Brasileira de Urologia, v. 40, n. 1, p. 72-79, 2014.
ISSN 1677-5538 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira de Urologia
Extent 72-79
Origin http://dx.doi.org/10.1590/S1677-5538.IBJU.2014.01.11
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000335430800012
SciELO ID S1677-55382014000100072 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8164

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