Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

Autor Lucon, Marcos Google Scholar
Martins, Joao Roberto Google Scholar
Leite, Kátia Ramos Moreira Autor UNIFESP Google Scholar
Soler, Roberto Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Srougi, Miguel Autor UNIFESP Google Scholar
Bruschini, Homero Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo Introduction: Painful bladder syndrome/interstitial cystitis (PBS/IC) pathogenesis is not fully known, but evidence shows that glycosaminoglycans (GAG) of bladder urothelium can participate in its genesis. The loss of these compounds facilitates the contact of urine compounds with deeper portions of bladder wall triggering an inflammatory process. We investigated GAG in urine and tissue of PBS/IC and pure stress urinary incontinence (SUI) patients to better understand its metabolism. Materials and Methods: Tissue and urine of 11 patients with PBS/IC according to NIDDK criteria were compared to 11 SUI patients. Tissue samples were analyzed by histological, immunohistochemistry and immunofluorescence methods. Statistical analysis were performed using t Student test and Anova, considering significant when p < 0.05. Results: PBS/IC patients had lower concentration of GAG in urine when compared to SUI (respectively 0.45 ± 0.11 x 0.62 ± 0.13 mg/mg creatinine, p < 0.05). However, there was no reduction of the content of GAG in the urothelium of both groups. Immunofluorescence showed that PBS/IC patients had a stronger staining of TGF-beta, decorin (a proteoglycan of chondroitin/dermatan sulfate), fibronectin and hyaluronic acid. Conclusion: the results suggest that GAG may be related to the ongoing process of inflammation and remodeling of the dysfunctional urothelium that is present in the PBS/IC.
Palavra-chave Cystitis, Interstitial
Glycosaminoglycans
Hyaluronic Acid
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Data de publicação 2014-01-01
Publicado em International braz j urol. Sociedade Brasileira de Urologia, v. 40, n. 1, p. 72-79, 2014.
ISSN 1677-5538 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira de Urologia
Extensão 72-79
Fonte http://dx.doi.org/10.1590/S1677-5538.IBJU.2014.01.11
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000335430800012
SciELO S1677-55382014000100072 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8164

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