Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats

Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats

Autor Freitas, André Luiz Pires de Autor UNIFESP Google Scholar
Silva, Marcelo de Oliveira e Google Scholar
Matsumoto, Priscilla Martin Keiko Google Scholar
Han, Sang Won Autor UNIFESP Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo PURPOSE: To investigate the experimental model for obtaining adipose tissue, isolation, characterization of mesenchymal stem cells and evaluation of their distribution in the tram flap in rats.METHODS: Five rats of Wistar were randomly assigned to two groups. In group I, three animals underwent removal of adipose tissue in the groin procedure to establish the experimental model and obtain a cell lineage. The animals of group II (n = 2) underwent surgical flap procedure, and satisfaction injection of mesenchymal stem cells pretreated with marker fluorescente.RESULTS: obtaining adipose tissue of the inguinal region of the rat proved to be possible. The isolated cells were characterized as mesenchymal stem cells and fluorescence microscopy showed the presence of multiple cells arranged around blood vessels and capillaries.CONCLUSION: It was possible to establish an experimental model for obtaining adipose tissue for isolation of mesenchymal stem cells and their distribution in the TRAM flap in rats.
Palavra-chave Stem Cells
Adipose Tissue
Animal Models
Mesenchymal Stem Cell Transplantation
Surgical Flaps
Idioma Inglês
Data de publicação 2014-01-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 29-33, 2014.
ISSN 0102-8650 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 29-33
Fonte http://dx.doi.org/10.1590/S0102-86502014001400006
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000342065300007
SciELO S0102-86502014001400007 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8146

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