Development of experimental in vitro burn model

Development of experimental in vitro burn model

Autor Fernandes, Ana Carolina Morais Google Scholar
França, Jerônimo Pereira de Autor UNIFESP Google Scholar
Gaiba, Silvana Google Scholar
Aloise, Antonio Carlos Autor UNIFESP Google Scholar
Oliveira, Andrea Fernandes de Google Scholar
Moraes, Andrea Aparecida Fátima Souza Autor UNIFESP Google Scholar
França, Lucimar Pereira de Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Santa Cruz Department of Biological Sciences
Resumo PURPOSE:To propose an experimental burn model in NIH-3T3 cell line.METHODS: Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS: The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.CONCLUSION: The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.
Palavra-chave Burn
fibroblast
cell line NIH-3T3
microwave
MTT
Confocal microscopy
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Data de publicação 2014-01-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 15-20, 2014.
ISSN 0102-8650 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 15-20
Fonte http://dx.doi.org/10.1590/S0102-86502014001400004
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000342065300005
SciELO S0102-86502014001400005 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8144

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