N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats

N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats

Author Amorim, Elivânia Marques Gomes de Google Scholar
Damous, Luciana Lamarão Autor UNIFESP Google Scholar
Durando, Maria Clara Silva Google Scholar
Saraiva, Márcia Viviane Alves Google Scholar
Koike, Marcia Kiyomi Google Scholar
Montero, Edna Frasson de Souza Autor UNIFESP Google Scholar
Institution Federal University do Vale do São Francisco
Universidade Federal de São Paulo (UNIFESP)
University of São Paulo Medical School Obstetrics and Gynecology Department
Federal University of Ceara Faculty of Medicine
Universidade de São Paulo (USP)
Abstract PURPOSE: To evaluate morphological and functional aspects of the ovarian graft in transplanted rats treated with NAC.METHODS: Female Wistar rats, virgin, 3 to 4 months old, weighing 200-250 grams were used in experiments. The rats have been kept in proper sanitary conditions, receiving food and water ad libitum. Five groups (n=10, each) were constituted: 4 groups treated subcutaneously with NAC, at doses of 150, 300, 600 and 1200 mg/kg (NAC150, NAC300, NAC600 and NAC1200, respectively), one hour of before the ovarian transplantation and control group (GTx) - treated with physiological solution and submitted to ovarian transplantation. The rats were anesthetized and submitted to autologous left ovarian transplantation, without anastomosis in retroperitoneum, and contralateral oophorectomy. During follow-up of 4 or 15 days, the estrous cycle was evaluated by vaginal smears to determine cycle regularity. At the end of 4th or 15th days, rats were re-anesthetized and blood and graft were obtained to estradiol analysis and morphological assessment. Data were analysed by One Way Analysis of Variance (ANOVA) or ANOVA on ranks complemented by Student-Newman-Keuls test.RESULTS: At 4th day, viable follicles in the graft did not altered by NAC treatments. The NAC300 and NAC600 groups showed increasing in follicle atresia (p=0.012) compared to GTx and NAC1200 group. At 15th day, 50% of GTx, NAC150, and NAC300 rats showed regular oestrous cycle; 83% of NAC600 and 100% of NAC1200 rats returned to regular cycle. NAC1200 group showed increasing in primordial follicle compared to GTx, NAC150 or NAC300 (p=0.011). NAC did not interfere in estradiol levels after 4 or 15 days of transplantation.CONCLUSION: In autologous ovarian transplantation, high dose of NAC promotes graft viability with recovery of estrous cycle.
Keywords Transplantation
Autologous
Acetylcysteine
Histology
Estrogen
Ovarium
Rats
Language English
Date 2014-01-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 22-27, 2014.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 22-27
Origin http://dx.doi.org/10.1590/S0102-86502014001700005
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000344466300006
SciELO ID S0102-86502014001500022 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8125

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