Expression of oxidative stress and antioxidant defense genes in the kidney of inbred mice after intestinal ischemia and reperfusion

Expression of oxidative stress and antioxidant defense genes in the kidney of inbred mice after intestinal ischemia and reperfusion

Autor Teruya, Roberto Autor UNIFESP Google Scholar
Ikejiri, Adauto Tsutomu Autor UNIFESP Google Scholar
Somaio Neto, Frederico Autor UNIFESP Google Scholar
Chaves, José Carlos Autor UNIFESP Google Scholar
Bertoletto, Paulo Roberto Google Scholar
Taha, Murched Omar Autor UNIFESP Google Scholar
Fagundes, Djalma José Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Mato Grosso do Sul Federal University Surgery Department
Grande Dourados Federal University Medical School
UFGD Medical School
Resumo PURPOSE: To determine the gene expressions profile related to the oxidative stress and the antioxidant response in the kidneys of mice subjected to intestinal ischemia and reperfusion. METHODS: Twelve inbred mice (C57BL/6) were randomly assigned to one of two groups: the control group (CG) underwent anesthesia and was observed for 120 min and the ischemia/reperfusion group (IRG), animals were anesthetized and subjected to laparotomy and ischemia for 60 minutes followed by 60 minutes of reperfusion. The expressions of 84 genes from the kidney were determined by the Reverse Transcription qualitative Polymerase Chain Reaction (RT-qPCR). All genes that were up regulated by more than threefold using the algorithm [2(ΔΔCt)] were considered statically significant (p<0.05). RESULTS: In the IRG group 29 (34.52%) of 84 genes, were up regulated by more than threefold. The genes that were differentially up regulated in the glutathione peroxidase cluster (10 genes): were Gpx2 and Gpx7. The genes that were up regulated in the peroxidase cluster (16 genes) were following: Duox1, Epx, Lpo, Mpo, Ptgs2, Rag2, Serpinb1b, Tmod1 and Tpo. The genes that up regulated in the reactive oxygen species cluster (16 genes): Il19, Il22, Nos2, Nox1, Noxa1, Noxo1, Recql4 and Sod2. The genes that were up regulated in the oxidative stress cluster (22 genes) were: Mpp4, Nudt15, Upc3 and Xpa. The genes that were up regulated in the oxygen carriers cluster (12 genes) were: Hbq1, Mb, Ngb, Slc38a1 and Xirp1. The peroxiredoxins genes (10) showed no consistent differential regulation. CONCLUSION: The genes related to oxidative stress and antioxidant defense showed increased expression in renal tissue trigged intestinal ischemia and reperfusion.
Palavra-chave Oxidative Stress
Gene Expression
Kidney
Reperfusion Injury
Mice
Idioma Inglês
Data de publicação 2013-12-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 12, p. 848-855, 2013.
ISSN 0102-8650 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 848-855
Fonte http://dx.doi.org/10.1590/S0102-86502013001200007
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000328649900007
SciELO S0102-86502013001200007 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8111

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