Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats

Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats

Autor Estevão, Lígia Reis Moura Google Scholar
Medeiros, Juliana Pinto de Google Scholar
Baratella-Evêncio, Liriane Google Scholar
Simões, Ricardo Santos Autor UNIFESP Google Scholar
Mendonça, Fábio de Souza Google Scholar
Evêncio-Neto, Joaquim Google Scholar
Instituição Federal Rural University of Pernambuco Department of Morphology and Animal Physiology Postgraduate Program in Animal Bioscience
UFRPE Department of Morphology and Physiology Histology Division
Federal University of Pernambuco Department of Morphology Histology Division
Universidade Federal de São Paulo (UNIFESP)
Resumo PURPOSE: To evaluate the effects of copaiba oil ointment (Copaifera langsdorffii) on dorsal skin flaps in rats. METHODS: Adult male rats (n=30) were distributed into three groups of ten animals each, as follows: GC - control; GCA - absolute control and GT - treated with copaiba ointment. The rats were subjected to dorsal cutaneous skin flap surgery and the animals from the GC and GT received post-operative treatment for eight consecutive days. The animals from the GCA group did not receive treatment while the animals from the GC group received daily topical treatment of ointment without the active ingredient and the animals from the GT group were daily treated with 10% copaiba oil ointment. At the end of each experimental period the lesions were evaluated according to the percentage of necrotic area. Then, fragments from cranial, median and caudal parts were fixed in Boüin's solution and processed for paraffin embedding. The morphology of histological sections (5µm) was evaluated and the number of leucocytes, fibroblasts and blood vessels was also analyzed. The data obtained were submitted to ANOVA test complemented by Tukey-Kramer test (p<0.05). RESULTS: The necrotic area was lower in the group treated with copaiba ointment when compared to the control groups (GCA>GC and GT), while the morphology showed larger granulation tissue with bulky fibroblasts and collagen fibers more arranged in the GT group. The morphometry showed a significant higher number of blood vessels in the median and caudal parts (GT>GCA and GC), leucocytes in the cranial part (GT>GC>GCA), and also fibroblasts in the median (GT and GC> GCA) and caudal parts (GT>GC and GCA) (p<0.05). CONCLUSION: The copaiba oil ointment favors angiogenesis and accelerates the viability of random skin flaps in rats.
Palavra-chave Surgical Flaps
Fabaceae
Skin
Ischemia
Rats
Idioma Inglês
Data de publicação 2013-12-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 12, p. 863-869, 2013.
ISSN 0102-8650 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 863-869
Fonte http://dx.doi.org/10.1590/S0102-86502013001200009
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000328649900009
SciELO S0102-86502013001200009 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8038

Exibir registro completo




Arquivo

Nome: S0102-86502013001200009.pdf
Tamanho: 1.515MB
Formato: PDF
Descrição:
Abrir arquivo

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta