Isolation and purification of bromelain from waste peel of pineapple for therapeutic application

Isolation and purification of bromelain from waste peel of pineapple for therapeutic application

Autor Bresolin, Iara Rocha Antunes Pereira Autor UNIFESP Google Scholar
Bresolin, Igor Tadeu Lazzarotto Autor UNIFESP Google Scholar
Silveira, Edgar Google Scholar
Tambourgi, Elias Basile Google Scholar
Mazzola, Priscila Gava Google Scholar
Instituição Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Uberlândia Instituto de Genética e Bioquímica
Resumo The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.
Palavra-chave Bromelain
pineapple peel
downstream processing
waste
therapeutic applications
enzymatic activity
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Data de publicação 2013-12-01
Publicado em Brazilian Archives of Biology and Technology. Instituto de Tecnologia do Paraná - Tecpar, v. 56, n. 6, p. 971-979, 2013.
ISSN 1516-8913 (Sherpa/Romeo, fator de impacto)
Publicador Instituto de Tecnologia do Paraná - Tecpar
Extensão 971-979
Fonte http://dx.doi.org/10.1590/S1516-89132013000600012
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000329451800012
SciELO S1516-89132013000600012 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/8026

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