Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients

Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients

Author Perosa, Ana Helena Sitta Autor UNIFESP Google Scholar
Watanabe, Aripuanã Sakurada Aranha Autor UNIFESP Google Scholar
Guatura, Sandra Baltazar Autor UNIFESP Google Scholar
Silva, Ellen Ricci Monteiro da Autor UNIFESP Google Scholar
Granato, Celso Francisco Hernandes Autor UNIFESP Google Scholar
Bellei, Nancy Cristina Junqueira Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVE: This study evaluated the diagnostic performance of two methods for the detection of influenza virus in immunocompromised transplant patients. METHODS: A total of 475 respiratory samples, 236 from patients in a hematopoietic stem cell transplantation program and 239 from kidney transplant patients, were analyzed by a direct fluorescence assay and the Centers for Disease Control real-time polymerase chain reaction protocol for influenza A and B detection. RESULTS: Influenza detection using either method was 7.6% in the hematopoietic stem cell transplant group and 30.5% in the kidney transplant patient group. Influenza detection by real-time polymerase chain reaction yielded a higher positive rate compared with fluorescence than that reported by other studies, and this difference was more pronounced for influenza A. The fluorescence assay sensitivity, specificity, positive and negative predictive values, and kappa coefficient were 17.6%, 100%, 1, 0.83, and 0.256, respectively, and lower detection rates occurred in the kidney transplant patients. CONCLUSIONS: The real-time polymerase chain reaction performance and the associated turnaround time for a large number of samples support the choice of this method for use in different routine diagnostic settings and influenza surveillance in high-risk patients.
Keywords Influenza Virus
Diagnostic Methods
Hematopoietic Stem Cell Transplant
Kidney Transplant Recipients
Language English
Sponsor Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
FAPESP
Date 2013-09-01
Published in Clinics. Faculdade de Medicina / USP, v. 68, n. 9, p. 1206-1209, 2013.
ISSN 1807-5932 (Sherpa/Romeo, impact factor)
1980-5322 (Sherpa/Romeo, impact factor)
Publisher Faculdade de Medicina / USP
Extent 1206-1209
Origin http://dx.doi.org/10.6061/clinics/2013(09)05
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000326016600005
SciELO ID S1807-59322013000901206 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7953

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