APC protein immunoexpression in colorectal adenoma and adenocarcinoma

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dc.contributor.author Bourroul, Vivian Sati Oba
dc.contributor.author Bourroul, Guilherme Muniz
dc.contributor.author Toloi, Giovanna Canato
dc.contributor.author Palma, Rogerio Tadeu
dc.contributor.author Oshima, Celina Tizuko Fujiyama [UNIFESP]
dc.contributor.author Gomes, Thiago Simao [UNIFESP]
dc.contributor.author Ihara, Silvia Saiuli Miki [UNIFESP]
dc.contributor.author Waisberg, Jaques
dc.date.accessioned 2015-06-14T13:45:36Z
dc.date.available 2015-06-14T13:45:36Z
dc.date.issued 2013-09-01
dc.identifier http://dx.doi.org/10.1016/j.jcol.2013.06.002
dc.identifier.citation Journal of Coloproctology (Rio de Janeiro). Sociedade Brasileira de Coloproctologia, v. 33, n. 3, p. 118-125, 2013.
dc.identifier.issn 2237-9363
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/7920
dc.description.abstract BACKGROUND:activation of the Wnt pathway by mutated APC gene is considered the initial event in colorectal carcinogenesis. The identification of these mutations can improve the specific treatment of the adenocarcinoma.OBJECTIVE:detect and evaluate wild-type APC protein in tissue from colorectal adenoma, adenocarcinoma and adjacent mucosa.METHODS:42 patients that underwent surgery for adenocarcinoma and 53 patients with resected adenomas were studied. Tissue samples from the adenocarcinoma were obtained from the tumor and from adjacent non-neoplastic mucosa located 10 cm from the proximal margin of the tumor. Adenoma tissue was obtained from representative areas. Blocks of tissue microarray (TMA) were submitted to immunohistochemistry with anti-APC, with readings of positivity and intensity of immunostaining and the score of immune expression of APC protein was obtained.RESULTS:the APC protein immune expression score showed a significantly lower expression of APC protein in the adenoma when compared with the adenocarcinoma (p < 0.0001) and adjacent mucosa (p < 0.0001). The APC protein immune expression score in the colorectal mucosa and adjacent to the adenocarcinoma showed no significant difference (p = 0.24).CONCLUSIONS:the finding of decreased expression of APC protein in adenoma tissue may indicate that the mutated APC gene may contribute to the changes in the adenoma-carcinoma process of carcinogenesis sequence. The strong expression of protein APC in tissues from the carcinoma and adjacent mucosa suggests that in most patients in this series, the mutation of the APC gene did not participate in the oncogenesis mechanism. en
dc.description.abstract RACIONAL:a ativação da via Wnt pelo gene APC mutado é considerado evento inicial da carcinogênese colorretal. A identificação dessas mutações pode tornar o tratamento do adenocarcinoma mais específico.OBJETIVO:detectar e avaliar a proteína APC não mutada em tecidos de adenoma, adenocarcinoma e mucosa adjacente.MÉTODO:estudados 42 doentes operados de adenocarcinoma e 53 com adenomas ressecados. Tecidos de adenocarcinoma foram obtidas da neoplasia e da mucosa adjacente não neoplásica situadas a 10 cm da margem proximal do tumor. Tecidos do adenoma foram obtidas de área representativa. Blocos de tissue microarray (TMA) foram submetidos a imuno-histoquímica com anticorpo anti-APC. Avaliadas a positividade e intensidade da expressão e obtidos escores da imunoexpressão da proteína APC.RESULTADOS:o escore da imunoexpressão da proteína APC no adenoma foi significantemente menor do que no adenocarcinoma (p < 0,0001) e na mucosa adjacente (p < 0,0001). O escore da imunoexpressão da proteína APC na mucosa adjacente e no adenocarcinoma não mostraram diferença significante (p = 0,24).CONCLUSÕES:a menor expressão da proteína APC no adenoma pode indicar que o gene APC mutado participa das alterações do processo adenoma-carcinoma. A forte expressão da proteína APC no CCR e na mucosa adjacente sugerem que a mutação do gene APC não participou da oncogênese. pt
dc.format.extent 118-125
dc.language.iso eng
dc.publisher Sociedade Brasileira de Coloproctologia
dc.relation.ispartof Journal of Coloproctology (Rio de Janeiro)
dc.rights Acesso aberto
dc.subject Colorectal neoplasms en
dc.subject Carcinoma en
dc.subject Adenoma en
dc.subject APC Gene en
dc.subject Immunohistochemistry en
dc.subject Biological tumor markers en
dc.subject Neoplasias colorretais pt
dc.subject Carcinoma pt
dc.subject Adenoma pt
dc.subject Gene APC pt
dc.subject Imunoistoquimica pt
dc.subject Marcadores biologicos de tumor pt
dc.title APC protein immunoexpression in colorectal adenoma and adenocarcinoma en
dc.title.alternative Imunoexpressao da proteina APC nos tecidos de adenoma e de adenocarcinoma colorretais pt
dc.type Artigo
dc.contributor.institution Hospital do Servidor Publico Estadual Service of Digestive System Surgery
dc.contributor.institution Hospital Estadual Mario Covas Service of Digestive System Surgery
dc.contributor.institution Faculdade de Medicina do ABC
dc.contributor.institution Faculdade de Medicina do ABC Service of Digestive System Surgery
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Faculdade de Medicina do ABC General and Digestive System Surgery
dc.description.affiliation Hospital do Servidor Publico Estadual Service of Digestive System Surgery
dc.description.affiliation Hospital Estadual Mario Covas Service of Digestive System Surgery
dc.description.affiliation Faculdade de Medicina do ABC
dc.description.affiliation Faculdade de Medicina do ABC Service of Digestive System Surgery
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Laboratory of Molecular Pathology
dc.description.affiliation UNIFESP-EPM Laboratory of Molecular Pathology
dc.description.affiliation Faculdade de Medicina do ABC General and Digestive System Surgery
dc.description.affiliationUnifesp UNIFESP, EPM, Laboratory of Molecular Pathology
dc.description.affiliationUnifesp UNIFESP, EPM Laboratory of Molecular Pathology
dc.identifier.file S2237-93632013000300118.pdf
dc.identifier.scielo S2237-93632013000300118
dc.identifier.doi 10.1016/j.jcol.2013.06.002
dc.description.source SciELO



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