Characterization of bacteriophages infecting clinical isolates of Pseudomonas aeruginosa stored in a culture collection

Characterization of bacteriophages infecting clinical isolates of Pseudomonas aeruginosa stored in a culture collection

Autor Zanetti, C.c.s. Google Scholar
Mingrone, R.c.c. Google Scholar
Kisielius, J.j. Google Scholar
Ueda-ito, M. Google Scholar
Pignatari, Antonio Carlos Campos Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Instituto Adolfo Lutz Nucleo de Microscopia Eletronica
Resumo Some clinical isolates of Pseudomonas aeruginosa stored in our culture collection did not grow or grew poorly and showed lysis on the culture plates when removed from the collection and inoculated on MacConkey agar. One hypothesis was that bacteriophages had infected and killed those clinical isolates. To check the best storage conditions to maintain viable P. aeruginosa for a longer time, clinical isolates were stored at various temperatures and were grown monthly. We investigated the presence of phage in 10 clinical isolates of P. aeruginosa stored in our culture collection. Four strains of P. aeruginosa were infected by phages that were characterized by electron microscopy and isolated to assess their ability to infect. The best condition to maintain the viability of the strains during storage was in water at room temperature. Three Siphoviridae and two Myoviridae phages were visualized and characterized by morphology. We confirmed the presence of bacteriophages infecting clinical isolates, and their ability to infect and lyse alternative hosts. Strain PAO1, however, did not show lysis to any phage. Mucoid and multidrug resistant strains of P. aeruginosa showed lysis to 50% of the phages tested.
Palavra-chave Bacteriophages
Pseudomonas aeruginosa
Idioma Inglês
Data de publicação 2013-08-13
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 46, n. 8, p. 689-695, 2013.
ISSN 0100-879X (Sherpa/Romeo)
Publicador Associação Brasileira de Divulgação Científica
Extensão 689-695
Fonte http://dx.doi.org/10.1590/1414-431X20132796
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0100-879X2013000800689 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/7911

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