Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis

Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis

Autor Matone, Jacques Autor UNIFESP Google Scholar
Moretti, Ana Iochabel Soares Google Scholar
Apodaca-Torrez, Franz Robert Autor UNIFESP Google Scholar
Goldenberg, Alberto Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo PURPOSE: To investigate if the ethyl-pyruvate solution could reduce mortality in AP and/or diminish the acute lung injury. METHODS: Forty male rats, weighing between 270 to 330 grams were operated. An experimental model of severe AP by injection of 0.1ml/100g of 2.5% sodium taurocholate into the bilio-pancreatic duct was utilized. The rats were divided into two groups of ten animals each: CT - control (treatment with 50ml/kg of Ringer's solution, intraperitoneal) and EP (treatment with 50ml/kg of Ringer ethyl- pyruvate solution, intra-peritoneal), three hours following AP induction. After six hours, a new infusion of the treatment solution was performed in each group. Two hours later, the animals were killed and the pulmonary parenchyma was resected for biomolecular analysis, consisting of: interleukin, myeloperoxidase, MDA, nitric oxide, metalloproteinases and heat shock protein. In the second part of the experiment, another, 20 rats were randomly divided into EP and CT groups, in order to evaluate a survival comparison between the two groups. RESULTS: There were no significant differences in IL-1B,IL-10, MMP-9, HSP70, nitric oxide, MPO, MDA (lipidic peroxidation) concerning both groups. The levels of IL-6 were significantly diminished in the EP group. Furthermore, the MMP-2 levels were also reduced in the EP group (p<0.05). The animals from the EP treatment groups had improved survival, when compared to control group (p<0.05). CONCLUSION: The ethyl-pyruvate diminishes acute lung injury inflammatory response in acute pancreatitis and ameliorates survival when compared to control group, in the experimental model of necrotizing acute pancreatitis.
Palavra-chave Pancreatitis
Acute Necrotizing
Pyruvic Acid
Inflammation
Rats
Idioma Inglês
Data de publicação 2013-08-01
Publicado em Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 8, p. 559-567, 2013.
ISSN 0102-8650 (Sherpa/Romeo)
Publicador Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extensão 559-567
Fonte http://dx.doi.org/10.1590/S0102-86502013000800002
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0102-86502013000800002 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/7890

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