Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection

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dc.contributor.author Watanabe, Aripuanã Sakurada Aranha [UNIFESP]
dc.contributor.author Carraro, Emerson [UNIFESP]
dc.contributor.author Camargo, Clarice Neves [UNIFESP]
dc.contributor.author Puerari, Diane
dc.contributor.author Guatura, Sandra
dc.contributor.author Granato, Celso Francisco Hernandes [UNIFESP]
dc.contributor.author Bellei, Nancy Cristina Junqueira [UNIFESP]
dc.date.accessioned 2015-06-14T13:45:24Z
dc.date.available 2015-06-14T13:45:24Z
dc.date.issued 2013-04-09
dc.identifier http://dx.doi.org/10.1590/0037-8682-1699-2013
dc.identifier.citation Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 2, p. 161-165, 2013.
dc.identifier.issn 0037-8682
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/7732
dc.description.abstract INTRODUCTION: Human adenoviruses (HAdV) play an important role in the etiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was detect the HAdV through different methods: direct fluorescence assay (DFA) and nested-polymerase chain reaction (PCR-nested) from patients with acute respiratory infection (ARI) up to 7 days of symptoms onset. METHODS: Samples (n=643) were collected from different risk groups during from 2001 to 2010: 139 adults attended in an Emergency Room Patients (ERP); 205 health care workers (HCW); 69 from Renal Transplant Outpatients (RTO); 230 patients in hematopoietic stem cell transplantation (HSCT) program. RESULTS: Among all patients (n=643) adenovirus was detected on 13.2% by DFA and/or PCR: 6/139 (4.3%) adults from ERP, 7/205 (3.4%) from HCW samples, 4/69 (5.8%) from RTO and 68/230 (29.5%) from HSCT patients. Nested PCR showed higher detection (10%) compared to DFA test (3.8%) (p < 0.001). HSCT patients presented significantly higher prevalence of HAdV infection. CONCLUSIONS: Adenovirus detection through nested-PCR assay was higher. However the inclusion of molecular method in laboratorial routine diagnostic should be evaluated considering the reality of each specific health service. en
dc.format.extent 161-165
dc.language.iso eng
dc.publisher Sociedade Brasileira de Medicina Tropical - SBMT
dc.relation.ispartof Revista da Sociedade Brasileira de Medicina Tropical
dc.rights Acesso aberto
dc.subject HAdV en
dc.subject Respiratory infection en
dc.subject DFA en
dc.subject Nested PCR en
dc.subject Immunocompromised patients and immunocompetent patients en
dc.title Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade do Centro-Oeste Departamento de farmacologia Clínica
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Departamento de Medicina Disciplina de Infectologia
dc.description.affiliation Universidade do Centro-Oeste Departamento de farmacologia Clínica
dc.description.affiliationUnifesp UNIFESP, Depto. de Medicina Disciplina de Infectologia
dc.identifier.file S0037-86822013000200161.pdf
dc.identifier.scielo S0037-86822013000200161
dc.identifier.doi 10.1590/0037-8682-1699-2013
dc.description.source SciELO



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