Ischemic preconditioning and the gene expression of enteric endothelial cell biology of rats submitted to intestinal ischemia and reperfusion

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dc.contributor.author Taha, Murched Omar [UNIFESP]
dc.contributor.author Miranda-Ferreira, Regiane [UNIFESP]
dc.contributor.author Taha, Nabiha Saadi Abrahão [UNIFESP]
dc.contributor.author Monteiro, Hugo Pequeno [UNIFESP]
dc.contributor.author Caricati-Neto, Afonso [UNIFESP]
dc.contributor.author Oliveira-Júnior, Itamar Souza [UNIFESP]
dc.contributor.author Fagundes, Djalma José [UNIFESP]
dc.date.accessioned 2015-06-14T13:45:20Z
dc.date.available 2015-06-14T13:45:20Z
dc.date.issued 2013-03-01
dc.identifier http://dx.doi.org/10.1590/S0102-86502013000300002
dc.identifier.citation Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 3, p. 167-173, 2013.
dc.identifier.issn 0102-8650
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/7675
dc.description.abstract PURPOSE: To investigate the effects of ischemic preconditioning (IPC) on the expression of pro and anti-apoptotic genes in rat endothelial cells undergoing enteric ischemia (I) and reperfusion (R). METHODS: Thirty rats underwent clamping of the superior mesenteric vessels. Sham group (GS) laparotomy only; Ischemia (GI): intestinal ischemia (60 min); Ischemia and Reperfusion (GIR): ischemia (60 min) and reperfusion (120 min); Ischemia and intestinal ischemic preconditioning (GI + IPC) : 5 minutes of ischemia followed by 10 min of reperfusion before sustained ischemia (60 min) ischemia and reperfusion and IPC (GIR + IPC): 5 min ischemia followed by 10 min of reperfusion before sustained ischemia (60min) and reperfusion (120 min). Rat Endothelial Cell Biology (PCR array) to determine the expression of genes related to endothelial cell biology. RESULTS: Gene expression of pro-apoptotic markers (Casp1, Casp6, Cflar, Fas, and Pgl) was down regulated in GI+IPC and in GIR + IPC. In contrast, the expression of anti-apoptotic genes (Bcl2 and Naip2), was up-regulated in GI + IPC and in GIR + IPC. CONCLUSION: Ischemic preconditioning may protect against cell death caused by ischemia and reperfusion. en
dc.format.extent 167-173
dc.language.iso eng
dc.publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartof Acta Cirurgica Brasileira
dc.rights Acesso aberto
dc.subject Ischemia en
dc.subject Reperfusion Injury en
dc.subject Endothelial Cells en
dc.subject Gene Expression Profiling en
dc.subject Rats en
dc.title Ischemic preconditioning and the gene expression of enteric endothelial cell biology of rats submitted to intestinal ischemia and reperfusion en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation UNIFESP
dc.description.affiliationUnifesp UNIFESP
dc.identifier.file S0102-86502013000300002.pdf
dc.identifier.scielo S0102-86502013000300002
dc.identifier.doi 10.1590/S0102-86502013000300002
dc.description.source SciELO
dc.identifier.wos WOS:000316126000002



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