Ischemic preconditioning and the gene expression of enteric endothelial cell biology of rats submitted to intestinal ischemia and reperfusion

Ischemic preconditioning and the gene expression of enteric endothelial cell biology of rats submitted to intestinal ischemia and reperfusion

Author Taha, Murched Omar Autor UNIFESP Google Scholar
Miranda-Ferreira, Regiane Autor UNIFESP Google Scholar
Taha, Nabiha Saadi Abrahão Autor UNIFESP Google Scholar
Monteiro, Hugo Pequeno Autor UNIFESP Google Scholar
Caricati-Neto, Afonso Autor UNIFESP Google Scholar
Oliveira-Júnior, Itamar Souza Autor UNIFESP Google Scholar
Fagundes, Djalma José Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE: To investigate the effects of ischemic preconditioning (IPC) on the expression of pro and anti-apoptotic genes in rat endothelial cells undergoing enteric ischemia (I) and reperfusion (R). METHODS: Thirty rats underwent clamping of the superior mesenteric vessels. Sham group (GS) laparotomy only; Ischemia (GI): intestinal ischemia (60 min); Ischemia and Reperfusion (GIR): ischemia (60 min) and reperfusion (120 min); Ischemia and intestinal ischemic preconditioning (GI + IPC) : 5 minutes of ischemia followed by 10 min of reperfusion before sustained ischemia (60 min) ischemia and reperfusion and IPC (GIR + IPC): 5 min ischemia followed by 10 min of reperfusion before sustained ischemia (60min) and reperfusion (120 min). Rat Endothelial Cell Biology (PCR array) to determine the expression of genes related to endothelial cell biology. RESULTS: Gene expression of pro-apoptotic markers (Casp1, Casp6, Cflar, Fas, and Pgl) was down regulated in GI+IPC and in GIR + IPC. In contrast, the expression of anti-apoptotic genes (Bcl2 and Naip2), was up-regulated in GI + IPC and in GIR + IPC. CONCLUSION: Ischemic preconditioning may protect against cell death caused by ischemia and reperfusion.
Keywords Ischemia
Reperfusion Injury
Endothelial Cells
Gene Expression Profiling
Rats
Language English
Date 2013-03-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 3, p. 167-173, 2013.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 167-173
Origin http://dx.doi.org/10.1590/S0102-86502013000300002
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000316126000002
SciELO ID S0102-86502013000300002 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7675

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