A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis

A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis

Autor Moreira, Luciana Peniche Autor UNIFESP Google Scholar
Watanabe, Aripuanã Sakurada Aranha Autor UNIFESP Google Scholar
Carraro, Emerson Autor UNIFESP Google Scholar
Silva, Ellen Ricci Monteiro da Autor UNIFESP Google Scholar
Guatura, Sandra Baltazar Autor UNIFESP Google Scholar
Granato, Celso Francisco Hernandes Autor UNIFESP Google Scholar
Bellei, Nancy Cristina Junqueira Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual do Centro Oeste
Resumo Human respiratory syncytial virus (HRSV) causes severe infections among children and immunocompromised patients. We compared HRSV infections among Haematopoietic Stem Cell Transplant program (HSCT) patients and children using direct immunofluorescence (DFA), point-of-care RSV Bio Easy® and a polymerase chain reaction (PCR) assay. Overall, 102 samples from HSCT patients and 128 from children obtained positivity rate of 18.6% and 14.1% respectively. PCR sensitivity was highest mainly on samples collected after five days of symptoms onset. A combination of both DFA and reverse transcriptase-PCR methods for HSCT high-risk patients is the best diagnostic flow for HRSV diagnosis among these patients.
Palavra-chave respiratory syncytial virus
comparison methods
human stem cell transplant patients
Idioma Inglês
Data de publicação 2013-02-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 108, n. 1, p. 119-122, 2013.
ISSN 0074-0276 (Sherpa/Romeo, fator de impacto)
Publicador Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 119-122
Fonte http://dx.doi.org/10.1590/S0074-02762013000100021
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000315335800021
SciELO S0074-02762013000100021 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/7615

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