Cytogenetics and sperm ultrastructure of Atelopus spumarius (Anura, Bufonidae) from the Brazilian Amazon

Cytogenetics and sperm ultrastructure of Atelopus spumarius (Anura, Bufonidae) from the Brazilian Amazon

Author Siqueira, Sérgio Google Scholar
Aguiar Junior, Odair Autor UNIFESP Google Scholar
Lima, Albertina Pimentel Google Scholar
Recco-pimentel, Shirlei Maria Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual do Sudoeste da Bahia Departamento de Ciências Biológicas
Universidade Federal de São Paulo (UNIFESP)
Instituto Nacional de Pesquisas da Amazônia Coordenadoria de Pesquisa em Ecologia
Abstract The current taxonomy of most Atelopus species is based on morphological and color data only. Recent studies suggest that A. spumarius may represent a species complex assigned under the same name. Karyotypic data and description of sperm ultrastructure for 13 specimens of A. spumarius are presented here for the first time. A chromosomal analysis revealed 2n = 22 chromosomes, with centromeric heterochromatin in all pairs and a nucleolar organizer region (NOR) on the telomere of pair 7. The sperm was of the bufonoid type, presenting a filiform nucleus covered by an acrosomal complex and a mitochondrial collar in the neck region. The tail was composed of an axoneme, an undulating membrane and an axial rod. A karyotype analysis of A. spumarius showed the same chromosome number and similar chromosomal morphology as described for congeneric species, with slight differences probably resulting from pericentric inversions. The NOR location (on pair 7) was the same as that observed for species belonging to the genus Rhinella. The spermatological findings indicate a close relationship between Atelopus and the bufonoid lineage. The present data are useful for reference in future studies to determine whether more than one species are assigned to A. spumarius.
Keywords Ag-NOR
Atelopus spumarius
C-band
chromosome
sperm ultrastructure
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2013-01-01
Published in Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 36, n. 4, p. 528-532, 2013.
ISSN 1415-4757 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira de Genética
Extent 528-532
Origin http://dx.doi.org/10.1590/S1415-47572013005000038
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000328702800011
WOS:000328702800011
SciELO ID S1415-47572013000400011 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7583

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