Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil

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dc.contributor.author Gelatti, Luciane Cristina
dc.contributor.author Bonamigo, Renan Rangel
dc.contributor.author Inoue, Fernanda Matsiko [UNIFESP]
dc.contributor.author Carmo, Mirian Silva do [UNIFESP]
dc.contributor.author Becker, Ana Paula
dc.contributor.author Castrucci, Fernanda Marques da Silvan [UNIFESP]
dc.contributor.author Pignatari, Antonio Carlos Campos [UNIFESP]
dc.contributor.author d'Azevedo, Pedro Alves [UNIFESP]
dc.date.accessioned 2015-06-14T13:45:11Z
dc.date.available 2015-06-14T13:45:11Z
dc.date.issued 2013-01-01
dc.identifier http://dx.doi.org/10.1590/0037-868213022013
dc.identifier.citation Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 1, p. 34-38, 2013.
dc.identifier.issn 0037-8682
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/7534
dc.description.abstract INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil. en
dc.format.extent 34-38
dc.language.iso eng
dc.publisher Sociedade Brasileira de Medicina Tropical - SBMT
dc.relation.ispartof Revista da Sociedade Brasileira de Medicina Tropical
dc.rights Acesso aberto
dc.subject Staphylococcus aureus en
dc.subject Methicillin-resistant en
dc.subject Skin infections en
dc.subject Soft tissue infections en
dc.title Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil en
dc.type Artigo
dc.contributor.institution Universidade Federal de Ciências da Saúde de Porto Alegre Laboratório de Cocos Gram-positivos
dc.contributor.institution Faculdade Serra da Mesa
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de Ciências da Saúde de Porto Alegre Laboratório de Cocos Gram-positivos
dc.description.affiliation Faculdade Serra da Mesa
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Laboratório Especial de Microbiologia Clínica
dc.description.affiliationUnifesp UNIFESP, Laboratório Especial de Microbiologia Clínica
dc.identifier.file S0037-86822013000100034.pdf
dc.identifier.scielo S0037-86822013000100034
dc.identifier.doi 10.1590/0037-868213022013
dc.description.source SciELO



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