Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil

Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil

Author Gelatti, Luciane Cristina Google Scholar
Bonamigo, Renan Rangel Google Scholar
Inoue, Fernanda Matsiko Autor UNIFESP Google Scholar
Carmo, Mirian Silva do Autor UNIFESP Google Scholar
Becker, Ana Paula Google Scholar
Castrucci, Fernanda Marques da Silvan Autor UNIFESP Google Scholar
Pignatari, Antonio Carlos Campos Autor UNIFESP Google Scholar
d'Azevedo, Pedro Alves Autor UNIFESP Google Scholar
Institution Universidade Federal de Ciências da Saúde de Porto Alegre Laboratório de Cocos Gram-positivos
Faculdade Serra da Mesa
Universidade Federal de São Paulo (UNIFESP)
Abstract INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil.
Keywords Staphylococcus aureus
Methicillin-resistant
Skin infections
Soft tissue infections
Language English
Date 2013-01-01
Published in Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 1, p. 34-38, 2013.
ISSN 0037-8682 (Sherpa/Romeo)
Publisher Sociedade Brasileira de Medicina Tropical - SBMT
Extent 34-38
Origin http://dx.doi.org/10.1590/0037-868213022013
Access rights Open access Open Access
Type Article
SciELO ID S0037-86822013000100034 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7534

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