Evaluation of rapid tests for human immunodeficiency virus as a tool to detect recent seroconversion

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dc.contributor.author Girardi, Samira Barboza [UNIFESP]
dc.contributor.author Barreto, Angela Maria Egydio de Carvalho
dc.contributor.author Barreto, Claudia Cortese
dc.contributor.author Proietti, Anna Barbara
dc.contributor.author Carvalho, Silvia Maia Farias de
dc.contributor.author Loureiro, Paula
dc.contributor.author Sabino, Ester Cerdeira [UNIFESP]
dc.date.accessioned 2015-06-14T13:44:59Z
dc.date.available 2015-06-14T13:44:59Z
dc.date.issued 2012-10-01
dc.identifier http://dx.doi.org/10.1016/j.bjid.2012.08.013
dc.identifier.citation Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 16, n. 5, p. 452-456, 2012.
dc.identifier.issn 1413-8670
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/7331
dc.description.abstract The identification of recent HIV infection is important for epidemiological studies and to monitor the epidemic. The objective of this study was to evaluate two rapid tests that are easily available to the Brazilian scientific community for using as markers of recent HIV infection. The Rapid Test - HIV-1/2 Bio-Manguinhos (Bio-Manguinhos/Fiocruz, Brazil) and the Rapid Check HIV 1&2 (NDI-UFES, Center for Infectious Diseases, Universidade Federal do Espírito Santo) were tested, using 489 samples with HIV positive serology, from blood donors, previously classified as recent or long-term infection by serological testing algorithm for recent HIV seroconversion (STARHS) or LS-HIV Vitros assay methods. The samples were diluted prior to testing (1:50 and 1:100 for the Rapid Test - HIV-1/2 Bio-Manguinhos, and 1:500 and 1:600 for the Rapid Check HIV 1&2). Negative samples were considered recent infection, whereas those showing any color intensity were associated with long-term infection. The best dilutions were 1:100 for HIV-1/2 Bio-Manguinhos test (Kappa = 0.840; overall agreement = 0.93), and 1:500 for the Rapid Check HIV 1&2 (Kappa = 0.867; overall agreement = 0.94). The results suggest that both rapid tests can be used to detect recent seroconversion. en
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 452-456
dc.language.iso eng
dc.publisher Brazilian Society of Infectious Diseases
dc.relation.ispartof Brazilian Journal of Infectious Diseases
dc.rights Acesso aberto
dc.subject HIV-1 incidence en
dc.subject Detuned assay en
dc.subject Surveillance en
dc.subject Rapid test en
dc.title Evaluation of rapid tests for human immunodeficiency virus as a tool to detect recent seroconversion en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Fundação Pró-Sangue Hemocentro de São Paulo
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Fundação Centro de Hematologia e Hemoterapia de Minas Gerais
dc.contributor.institution Faculdade de Saúde e Ecologia Humana
dc.contributor.institution Instituto Estadual de Hematologia Arthur de Siqueira Cavalcanti
dc.contributor.institution Fundação Hemope
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina
dc.description.affiliation Fundação Pró-Sangue Hemocentro de São Paulo
dc.description.affiliation FMUSP Department of Molecular Biology
dc.description.affiliation Fundação Centro de Hematologia e Hemoterapia de Minas Gerais
dc.description.affiliation Faculdade de Saúde e Ecologia Humana
dc.description.affiliation Instituto Estadual de Hematologia Arthur de Siqueira Cavalcanti
dc.description.affiliation Fundação Hemope
dc.description.affiliation Universidade de São Paulo Institute of Tropical Medicine Department of Infectious Disease
dc.description.affiliationUnifesp UNIFESP, EPM
dc.identifier.file S1413-86702012000500009.pdf
dc.identifier.scielo S1413-86702012000500009
dc.identifier.doi 10.1016/j.bjid.2012.08.013
dc.description.source SciELO
dc.identifier.wos WOS:000310088400009



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