Metallo-β-lactamase-production in meropenem-susceptible Pseudomonas aeruginosa isolates: risk for silent spread

Metallo-β-lactamase-production in meropenem-susceptible Pseudomonas aeruginosa isolates: risk for silent spread

Autor Picão, Renata Cristina Autor UNIFESP Google Scholar
Carrara-marroni, Floristher Elaine Google Scholar
Gales, Ana Cristina Autor UNIFESP Google Scholar
Venâncio, Emerson José Google Scholar
Xavier, Danilo Elias Autor UNIFESP Google Scholar
Tognim, Maria Cristina Bronharo Autor UNIFESP Google Scholar
Pelayo, Jacinta Sanchez Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro Departamento de Microbiologia Médica
Universidade Estadual de Maringá Departamento de Patologia, Análises Clínicas e Toxicologia
Universidade Estadual de Maringá Departamento de Ciências Patológicas
Universidade Estadual de Maringá Departamento de Ciências Básicas da Saúde
Universidade Estadual de Londrina Departamento de Microbiologia
Resumo The aim of this study was to characterize two metallo-β-lactamases (MBLs)-producing Pseudomonas aeruginosa clinical isolates showing meropenem susceptibility. Antimicrobial susceptibility was assessed by automated testing and Clinical and Laboratory Standards Institute agar dilution method. MBL production was investigated by phenotypic tests. Molecular typing was determined by pulsed field gel electrophoresis (PFGE). MBL-encoding genes, as well as their genetic context, were identified by polymerase chain reaction (PCR) and sequencing. The location of blaIMP-16 was determined by plasmid electrophoresis, Southern blot and hybridization. Transcriptional levels of blaIMP-16, mexB, mexD, mexF, mexY, ampC and oprD were determined by semi-quantitative real time PCR. The P. aeruginosa isolates studied, Pa30 and Pa43, showed imipenem and meropenem susceptibility by automated testing. Agar dilution assays confirmed meropenem susceptibility whereas both isolates showed low level of imipenem resistance. Pa30 and Pa43 were phenotypically detected as MBL producers. PFGE revealed their clonal relatedness. blaIMP-16 was identified in both isolates, carried as a single cassette in a class 1 integron that was embedded in a plasmid of about 60-Kb. Pa30 and Pa43 overexpressed MexAB-OprM, MexCD-OprJ and MexXY-OprM efflux systems and showed basal transcriptional levels of ampC and oprD. MBL-producing P. aeruginosa that are not resistant to meropenem may represent a risk for therapeutic failure and act as silent reservoirs of MBL-encoding genes.
Palavra-chave Beta-lactam resistance
Beta-lactamase
meropenem
efflux pumps
Gram-negative non-fermenting
Idioma Inglês
Data de publicação 2012-09-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 107, n. 6, p. 747-751, 2012.
ISSN 0074-0276 (Sherpa/Romeo, fator de impacto)
Publicador Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 747-751
Fonte http://dx.doi.org/10.1590/S0074-02762012000600007
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000308943900007
SciELO S0074-02762012000600007 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/7289

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