Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein

Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein

Author Passos, E.p. Google Scholar
Brugnara, L. Google Scholar
Facin, A.c. Google Scholar
Riffel, A. Google Scholar
Lima, G.r. Autor UNIFESP Google Scholar
Freitas, V. Autor UNIFESP Google Scholar
Brandelli, A. Google Scholar
Institution Hospital de Clínicas de Porto Alegre
Universidade Federal de São Paulo (UNIFESP)
Abstract The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11%) and PFe/PFi groups (17%), and the ionophore-induced AR was higher for PFi (33%) than PFc/PFe (25%). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR.
Keywords acrosome reaction
endometriosis
infertility
peritoneal fluid
sperm
Language English
Date 1999-01-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 1, p. 59-65, 1999.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 59-65
Origin http://dx.doi.org/10.1590/S0100-879X1999000100009
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000078324100009
SciELO ID S0100-879X1999000100009 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/725

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