Rhinovirus detection using different PCR-based strategies

Rhinovirus detection using different PCR-based strategies

Autor Silva, Ellen Ricci Monteiro da Autor UNIFESP Google Scholar
Watanabe, Aripuanã Sakurada Aranha Autor UNIFESP Google Scholar
Carraro, Emerson Autor UNIFESP Google Scholar
Perosa, Ana Helena Sitta Autor UNIFESP Google Scholar
Granato, Celso Francisco Hernandes Autor UNIFESP Google Scholar
Bellei, Nancy Cristina Junqueira Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Human rhinoviruses (HRVs) are the major cause of the common cold. HRVs were recently reclassified into the Enterovirus genus (HEV) in the Picornaviridae family. HRVs and other members of the HEV genus share many common features, including sense RNA genomes and partial nucleotide sequence identity. The aim of this study was to evaluate different HRV detection strategies. Samples from adults with acute respiratory infection (n = 291) who were treated in São Paulo Hospital (2001-2003) were tested using three assays. The first assay detected picornaviruses by RT-PCR and hybridization, the second detected rhinoviruses using RT-PCR/sequencing, and the third differentiated HRV from HEV using duplex semi-nested-RT-PCR. Analysis of the results obtained from the first two strategies revealed 83% concordance. Discordant samples were then evaluated by the third protocol, and 82% were negative. The picornavirus detection protocol was more sensitive but less specific than the rhinovirus detection protocols. The semi-nested protocol utilized in the present study was less sensitive and was not useful in differentiating HRV from HEV. Sequencing assays examining different genes would address the best strategy of confirming rhinovirus and enterovirus infections.
Palavra-chave Rhinovirus
Enterovirus
Detection
Differentiation
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Data de publicação 2012-06-01
Publicado em Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 43, n. 2, p. 739-743, 2012.
ISSN 1517-8382 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira de Microbiologia
Extensão 739-743
Fonte http://dx.doi.org/10.1590/S1517-83822012000200038
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000307438100038
SciELO S1517-83822012000200038 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/7176

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