A liver metalloendopeptidase which degrades the circulating hypotensive peptide hormones bradykinin and atrial natriuretic peptide

A liver metalloendopeptidase which degrades the circulating hypotensive peptide hormones bradykinin and atrial natriuretic peptide

Autor Carvalho, K.m. Google Scholar
Nava, R.a. Google Scholar
França, M.s.f. Google Scholar
Medeiros, M.a.s. Google Scholar
Camarão, Gisela Costa Autor UNIFESP Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Instituição Universidade Federal do Ceará
Universidade Estadual do Ceará
Universidade Federal de São Paulo (UNIFESP)
Resumo A new metalloendopeptidase was purified to apparent homogeneity from a homogenate of normal human liver using successive steps of chromatography on DEAE-cellulose, hydroxyapatite and Sephacryl S-200. The purified enzyme hydrolyzed the Pro7-Phe8 bond of bradykinin and the Ser25-Tyr26 bond of atrial natriuretic peptide. No cleavage was produced in other peptide hormones such as vasopressin, oxytocin or Met- and Leu-enkephalin. This enzyme activity was inhibited by 1 mM divalent cation chelators such as EDTA, EGTA and o-phenanthroline and was insensitive to 1 µM phosphoramidon and captopril, specific inhibitors of neutral endopeptidase (EC 3.4.24.11) and angiotensin-converting enzyme (EC 3.4.15.1), respectively. With Mr 85 kDa, the enzyme exhibits optimal activity at pH 7.5. The high affinity of this endopeptidase for bradykinin (Km = 10 µM) and for atrial natriuretic peptide (Km = 5 µM) suggests that it may play a physiological role in the inactivation of these circulating hypotensive peptide hormones.
Palavra-chave liver metalloendopeptidase
bradykinin
atrial natriuretic peptide
Idioma Inglês
Data de publicação 1999-01-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 1, p. 51-54, 1999.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 51-54
Fonte http://dx.doi.org/10.1590/S0100-879X1999000100007
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000078324100007
SciELO S0100-879X1999000100007 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/712

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