Characterization of anti-silencing factor 1 in Leishmania major

Characterization of anti-silencing factor 1 in Leishmania major

Autor Scher, Ricardo Google Scholar
Garcia, Juliana Bório Ferreira Google Scholar
Pascoalino, Bruno Autor UNIFESP Google Scholar
Schenkman, Sergio Autor UNIFESP Google Scholar
Cruz, Angela Kaysel Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de Sergipe Centro de Ciências Biológicas e da Saúde Departamento de Morfologia
Universidade Federal de São Paulo (UNIFESP)
Resumo Anti-silencing factor 1 (ASF1) is a histone chaperone that contributes to the histone deposition during nucleosome assembly in newly replicated DNA. It is involved in chromatin disassembly, transcription activation and in the cellular response to DNA damage. In Leishmania major the ASF1 gene (LmASF1) is located in chromosome 20 and codes for a protein showing 67% of identity with the Trypanosoma brucei TbASF1a. Compared to orthologous proteins, LmASF1 conserves the main residues relevant for its various biological functions. To study ASF1 in Leishmania we generated a mutant overexpressing LmASF1 in L. major. We observed that the excess of LmASF1 impaired promastigotes growth rates and had no impact on cell cycle progress. Differently from yeast, ASF1 overproduction in Leishmania did not affect expression levels of genes located on telomeres, but led to an upregulation of proteins involved in chromatin remodelling and physiological stress, such as heat shock proteins, oxidoreductase activity and proteolysis. In addition, we observed that LmASF1 mutant is more susceptible to the DNA damaging agent, methyl methane sulphonate, than the control line. Therefore, our study suggests that ASF1 from Leishmania pertains to the chromatin remodelling machinery of the parasite and acts on its response to DNA damage.
Assunto Leishmania major
anti-silencing factor 1
histone chaperone
Idioma Inglês
Data 2012-05-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 107, n. 3, p. 377-386, 2012.
ISSN 0074-0276 (Sherpa/Romeo, fator de impacto)
Editor Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 377-386
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000302933000013
SciELO S0074-02762012000300013 (estatísticas na SciELO)

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