Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats

Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats

Author Kracochansky, Mirian Autor UNIFESP Google Scholar
Reis, Leonardo Oliveira Google Scholar
Lorenzetti, Fábio Autor UNIFESP Google Scholar
Ortiz, Valdemar Autor UNIFESP Google Scholar
Dambros, Miriam Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Abstract OBJECTIVE: To analyze the impact of low levels of testosterone induced by orchiectomy and the effect of alpha-tocopherol supplementation on oxidative stress in the urethral sphincter. MATERIALS AND METHODS: Forty male Wistar rats weighing 250-300g were divided into four groups with 10 each: Sham group; Orchiectomy group: bilateral orchiectomy; Orchiectomy-pre-Tocopherol group: bilateral orchiectomy preceded by alpha-tocopherol supplementation for four weeks; Orchiectomy-full-Tocopherol group: bilateral orchiectomy with alpha-tocopherol supplementation for four weeks preceding the procedure and for eight weeks afterwards. At the protocol end, animals were euthanized and had the sphincter analyzed stereologically focusing on collagen and muscle fibers percentage. Oxidative stress levels were determined using 8-epi-PGF2. RESULTS: The 8-epi-PGF2 levels were statistically higher (p < 0.0003) in the Orchiectomy group compared to others groups while Sham and Orchiectomy-full-Tocopherol groups presented statistically similar values (p = 0.52). Collagen volumetric densities were significantly lower in Sham and Orchiectomy-full-Tocopherol groups (p < 0.022). Sham group presented statistically greater muscle fiber percent. CONCLUSION: Castration caused oxidative stress in the urethral sphincter complex, with increased collagen deposition. Alpha-tocopherol had a protective effect and its supplementation for twelve weeks provided the greatest protection.
Keywords apoptosis
alpha-tocopherol
oxidative stress
antioxidants
castration
Language English
Date 2012-04-01
Published in International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 2, p. 277-283, 2012.
ISSN 1677-5538 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira de Urologia
Extent 277-283
Origin http://dx.doi.org/10.1590/S1677-55382012000200017
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000306550800033
SciELO ID S1677-55382012000200017 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7056

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