Tissue expression of CD10 protein in colorectal carcinoma: correlation with the anatomopathological features of the tumor and with lymph node and liver metastases

Tissue expression of CD10 protein in colorectal carcinoma: correlation with the anatomopathological features of the tumor and with lymph node and liver metastases

Author Oliveira, Levindo Alves de Autor UNIFESP Google Scholar
Artigiani Neto, Ricardo Autor UNIFESP Google Scholar
Joppert Netto, George Autor UNIFESP Google Scholar
Sznirer, Monica Autor UNIFESP Google Scholar
Fernandes, Luis Cesar Autor UNIFESP Google Scholar
Lima, Flávio de Oliveira Autor UNIFESP Google Scholar
Waisberg, Jaques Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract BACKGROUND: The reduced expression of CD10 may be related to unfavorable prognosis of patients with colorectal carcinoma. The authors analyzed the tissue immunostaining of CD10 protein in colorectal carcinoma and its relationship to clinicopathologic features. METHOD: In 130 patients submitted to colorectal carcinoma surgery, a tissue microarray block was obtained from the tumor and adjacent non-neoplastic mucosa and submitted to immunohistochemistry with monoclonal antibody CD10. The immunostaining was evaluated by semi-quantitative method, with stained cell count in percentage. The results were related to the location, anatomopathological features, presence of lymph node and hepatic metastases and TNM staging of the colorectal neoplasm. The statistical analysis was performed with the Mann-Whitney, Kruskal-Wallis and Fisher exact tests. RESULTS: The expression of CD10 marker was higher in colorectal tumor tissue than in adjacent non-neoplastic mucosa (p<0.0001) and was higher than in exophytic lesions (p=0.04). The expression of CD10 protein was not associated with other clinical and pathological aspects of colorectal neoplasm. CONCLUSIONS: The expression of CD10 protein was more intense in tumor tissue of colorectal carcinoma than in adjacent non-neoplastic mucosa and was related to the exophytic appearance of the tumor.

INTRODUÇÃO: A expressão reduzida de CD10 pode estar relacionada com prognóstico desfavorável de doentes com carcinoma colorretal. Analisou-se a imunoexpressão tecidual da proteína CD10 no carcinoma colorretal e sua relação com os aspectos clinicopatológicos. MÉTODO: Em 130 doentes operados por carcinoma colorretal, um bloco de tissue microarray foi obtido do tecido neoplásico e da mucosa não neoplásica adjacente e submetido ao estudo imuno-histoquímico com anticorpo monoclonal CD10. Avaliou-se a imunoexpressão por método semiquantitativo, com contagem do percentual de células coradas. Os resultados foram relacionados com a localização, aspectos anatomopatológicos, presença de metástases linfonodais e hepáticas e estadiamento TNM da neoplasia. O estudo estatístico foi realizado com os testes de Mann-Whitney, Kruskal-Wallis e exato de Fisher. RESULTADOS: A expressão do marcador CD10 foi maior no tecido do carcinoma colorretal do que na mucosa não neoplásica adjacente (p<0,0001) e foi maior nas lesões exofíticas (p=0,04). A expressão da proteína CD10 não apresentou relação com os demais aspetos clínicos e patológicos da neoplasia colorretal. CONCLUSÕES: A expressão da proteína CD10 foi mais intensa no tecido neoplásico do carcinoma colorretal do que na mucosa não neoplásica adjacente e relacionou-se com o aspecto exofítico do tumor.
Keywords Neoplasm antigens
Colorectal neoplasms
Prognosis
Neoplasm metastasis
CD10 antigens
Antígenos de neoplasia
Neoplasias colorretais
Prognóstico
Metástase neoplásica
Antígenos CD10
Language English
Date 2012-03-01
Published in Journal of Coloproctology (Rio de Janeiro). Sociedade Brasileira de Coloproctologia, v. 32, n. 1, p. 34-39, 2012.
ISSN 2237-9363 (Sherpa/Romeo)
Publisher Sociedade Brasileira de Coloproctologia
Extent 34-39
Origin http://dx.doi.org/10.1590/S2237-93632012000100005
Access rights Open access Open Access
Type Article
SciELO ID S2237-93632012000100005 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7005

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