Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation

Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation

Autor Skaff, M. Autor UNIFESP Google Scholar
Pinto, E.r.s. Autor UNIFESP Google Scholar
Leite, Kátia Ramos Moreira Autor UNIFESP Google Scholar
Almeida, F.g. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo OBJECTIVE: The aim of the study was to develop a new durable animal model (using rabbits) for anatomical-functional evaluation of urethral sphincter deficiency. MATERIALS AND METHODS: A total of 40 New Zealand male rabbits, weighting 2.500 kg to 3.100 kg, were evaluated to develop an incontinent animal model. Thirty-two animals underwent urethrolysis and 8 animals received sham operation. Before and at 2, 4, 8 and 12 weeks after urethrolysis or sham operation, it was performed cystometry and leak point pressure (LPP) evaluation with different bladder distension volumes (10, 20, 30 mL). In each time point, 10 animals (8 from the study group and 2 from the sham group) were sacrificed to harvest the bladder and urethra. The samples were evaluated by H&E and Masson's Trichrome to determine urethral morphology and collagen/smooth muscle density. RESULTS: Twelve weeks after urethrolysis, it was observed a significant decrease in LPP regardless the bladder volume (from 33.7 ± 6.6 to 12.8 ± 2.2 cmH2O). The histological analysis evidenced a decrease of 22% in smooth muscle density with a proportional increase in the collagen, vessels and elastin density (p < 0.01). CONCLUSIONS: Transabdominal urethrolysis develops urethral sphincter insufficiency in rabbits, with significant decrease in LPP associated with decrease of smooth muscle fibers and increase of collagen density. This animal model can be used to test autologous cell therapy for stress urinary incontinence treatment.
Palavra-chave Stem cells
tissue engineering
urinary incontinence
animal model
transabdominal urethrolysis
urethral sphincter deficiency
Idioma Inglês
Data de publicação 2012-02-01
Publicado em International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 1, p. 17-24, 2012.
ISSN 1677-5538 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira de Urologia
Extensão 17-24
Fonte http://dx.doi.org/10.1590/S1677-55382012000100003
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000301487700005
SciELO S1677-55382012000100003 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/6966

Exibir registro completo




Arquivo

Nome: S1677-55382012000100003.pdf
Tamanho: 403.5KB
Formato: PDF
Descrição:
Abrir arquivo

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta