Detection of Helicobacter pylori in gastric biopsies, saliva and dental plaques of dyspeptic patients from Marília, São Paulo, Brazil: presence of vacA and cagA genes

Detection of Helicobacter pylori in gastric biopsies, saliva and dental plaques of dyspeptic patients from Marília, São Paulo, Brazil: presence of vacA and cagA genes

Autor Rasmussen, Lucas Trevizani Autor UNIFESP Google Scholar
Lábio, Roger Willian de Google Scholar
Caleman Neto, Agostinho Google Scholar
Silva, Luiz Carlos da Google Scholar
Queiroz, Valdeir Fagundes de Google Scholar
Smith, Marilia de Arruda Cardoso Autor UNIFESP Google Scholar
Payão, Spencer Luiz Marques Autor UNIFESP Google Scholar
Instituição Sacred Heart University Postgraduate Program in Oral Biology
Marília Medical School Blood Center Department of Genetics
Marília Medical School Department of Anatomic Pathology
Marília Medical School Department of Digestive System Surgery
Universidade Federal de São Paulo (UNIFESP)
Resumo Helicobacter pylori, a gram-negative bacterium, possesses two important virulence factors: the vacuolating toxin (vacA), and the cytotoxin-associated gene product (cagA). The aim of the present study was to evaluate the presence of H. pylori in the stomach and oral cavity of humans and compare the cagA and vacA genotypes of H. pylori found in different samples (stomach, saliva and dental plaque) from the same patient. Gastric biopsies, saliva and dental plaques were obtained from 62 dyspeptic adults. DNA was extracted and evaluated for the presence of H. pylori and the alleles cagA and vacA. Persons with gastritis had a higher frequency of H. pylori -positive samples in the stomach while positive samples from gastric biopsies were significantly correlated with those from the oral cavity. There was a high H. pylori frequency in patients while the cagA gene was associated with vacA s1 alleles in gastric biopsies. Our results suggest a reservoir of the species in the oral cavity and that, in one patient, more than one H. pylori strain may exist in the saliva, dental plaque and stomach. We found a relationship between gastric infection and the bacterium in the oral cavity, with the cytotoxin genotype varying between saliva and dental plaque.
Palavra-chave Helicobacter pylori
cagA
vacA
dental plaque
dyspeptic patient
saliva
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Data de publicação 2012-01-01
Publicado em Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 18, n. 2, p. 180-187, 2012.
ISSN 1678-9199 (Sherpa/Romeo, fator de impacto)
Publicador Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Extensão 180-187
Fonte http://dx.doi.org/10.1590/S1678-91992012000200008
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000305761800008
SciELO S1678-91992012000200008 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/6896

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