Thymopoiesis and regulatory T cells in healthy children and adolescents

Thymopoiesis and regulatory T cells in healthy children and adolescents

Author Arismendi, Maria Izabel Google Scholar
Kallas, Esper Georges Autor UNIFESP Google Scholar
Santos, Bianca Almeida Natali dos Google Scholar
Carneiro-Sampaio, Magda Maria Sales Google Scholar
Kayser, Cristiane Autor UNIFESP Google Scholar
Institution Instituto da Criança Departamento de Pediatria
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVES: The purpose of this study was to investigate the association between T cell receptor excision circle levels in peripheral blood mononuclear cells and regulatory T cells that co-express CD25 and Foxp3 in healthy children and adolescents of different ages. MATERIALS AND METHODS: The quantification of signal-joint T-cell receptor excision circle levels in the genomic DNA of peripheral blood mononuclear cells was performed using real-time quantitative PCR. The analysis of CD4, CD8, CD25, and Foxp3 expression was performed using flow cytometry. RESULTS: Ninety-five healthy controls (46 females and 49 males) ranging in age from 1 to 18 years were analyzed. The mean T-cell receptor excision circle count in all individuals was 89.095¡36.790 T-cell receptor excision circles per microgram of DNA. There was an inverse correlation between T-cell receptor excision circles counts and age (r = -0.846; p<0.001) as well as between the proportion of CD4+CD25+Foxp3+ T cells and age (r = -0.467; p = 0.04). In addition, we observed a positive correlation between the amount of CD4+CD25+Foxp3+ T cells and the amount of Tcell receptor excision circles per microgram of DNA in individuals of all ages (r = -0.529; p = 0.02). CONCLUSIONS: In this study, we observed a decrease in the thymic function with age based on the fact that the level of T-cell receptor excision circles in the peripheral blood positively correlated with the proportion of regulatory T cells in healthy children and adolescents. These findings indicate that although T-cell receptor excision circles and regulatory T cells levels decrease with age, homeostasis of the immune system and relative regulatory T cells population levels are maintained in the peripheral blood.
Keywords T lymphocytes
Thymus
Foxp3
T-cell Receptor
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number 2008/58238-4
Date 2012-01-01
Published in Clinics. Faculdade de Medicina / USP, v. 67, n. 5, p. 425-429, 2012.
ISSN 1807-5932 (Sherpa/Romeo, impact factor)
Publisher Faculdade de Medicina / USP
Extent 425-429
Origin http://dx.doi.org/10.6061/clinics/2012(05)04
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000304827700004
SciELO ID S1807-59322012000500004 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/6806

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