Autor |
Arismendi, Maria Izabel
![]() Kallas, Esper Georges ![]() ![]() Santos, Bianca Almeida Natali dos ![]() Carneiro-Sampaio, Magda Maria Sales ![]() Kayser, Cristiane ![]() ![]() |
Instituição | Instituto da Criança Departamento de Pediatria Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
Resumo | OBJECTIVES: The purpose of this study was to investigate the association between T cell receptor excision circle levels in peripheral blood mononuclear cells and regulatory T cells that co-express CD25 and Foxp3 in healthy children and adolescents of different ages. MATERIALS AND METHODS: The quantification of signal-joint T-cell receptor excision circle levels in the genomic DNA of peripheral blood mononuclear cells was performed using real-time quantitative PCR. The analysis of CD4, CD8, CD25, and Foxp3 expression was performed using flow cytometry. RESULTS: Ninety-five healthy controls (46 females and 49 males) ranging in age from 1 to 18 years were analyzed. The mean T-cell receptor excision circle count in all individuals was 89.095¡36.790 T-cell receptor excision circles per microgram of DNA. There was an inverse correlation between T-cell receptor excision circles counts and age (r = -0.846; p<0.001) as well as between the proportion of CD4+CD25+Foxp3+ T cells and age (r = -0.467; p = 0.04). In addition, we observed a positive correlation between the amount of CD4+CD25+Foxp3+ T cells and the amount of Tcell receptor excision circles per microgram of DNA in individuals of all ages (r = -0.529; p = 0.02). CONCLUSIONS: In this study, we observed a decrease in the thymic function with age based on the fact that the level of T-cell receptor excision circles in the peripheral blood positively correlated with the proportion of regulatory T cells in healthy children and adolescents. These findings indicate that although T-cell receptor excision circles and regulatory T cells levels decrease with age, homeostasis of the immune system and relative regulatory T cells population levels are maintained in the peripheral blood. |
Palavra-chave |
T lymphocytes
Thymus Foxp3 T-cell Receptor |
Idioma | Inglês |
Financiador |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) |
Número do financiamento |
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Data de publicação | 2012-01-01 |
Publicado em | Clinics. Faculdade de Medicina / USP, v. 67, n. 5, p. 425-429, 2012. |
ISSN | 1807-5932 (Sherpa/Romeo, fator de impacto) |
Publicador | Faculdade de Medicina / USP |
Extensão | 425-429 |
Fonte |
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Direito de acesso | Acesso aberto ![]() |
Tipo | Artigo |
Web of Science | WOS:000304827700004 |
SciELO | S1807-59322012000500004 (estatísticas na SciELO) |
Endereço permanente | http://repositorio.unifesp.br/handle/11600/6806 |
Nome:
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