Thymopoiesis and regulatory T cells in healthy children and adolescents

Thymopoiesis and regulatory T cells in healthy children and adolescents

Autor Arismendi, Maria Izabel Google Scholar
Kallas, Esper Georges Autor UNIFESP Google Scholar
Santos, Bianca Almeida Natali dos Google Scholar
Carneiro-Sampaio, Magda Maria Sales Google Scholar
Kayser, Cristiane Autor UNIFESP Google Scholar
Instituição Instituto da Criança Departamento de Pediatria
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo OBJECTIVES: The purpose of this study was to investigate the association between T cell receptor excision circle levels in peripheral blood mononuclear cells and regulatory T cells that co-express CD25 and Foxp3 in healthy children and adolescents of different ages. MATERIALS AND METHODS: The quantification of signal-joint T-cell receptor excision circle levels in the genomic DNA of peripheral blood mononuclear cells was performed using real-time quantitative PCR. The analysis of CD4, CD8, CD25, and Foxp3 expression was performed using flow cytometry. RESULTS: Ninety-five healthy controls (46 females and 49 males) ranging in age from 1 to 18 years were analyzed. The mean T-cell receptor excision circle count in all individuals was 89.095¡36.790 T-cell receptor excision circles per microgram of DNA. There was an inverse correlation between T-cell receptor excision circles counts and age (r = -0.846; p<0.001) as well as between the proportion of CD4+CD25+Foxp3+ T cells and age (r = -0.467; p = 0.04). In addition, we observed a positive correlation between the amount of CD4+CD25+Foxp3+ T cells and the amount of Tcell receptor excision circles per microgram of DNA in individuals of all ages (r = -0.529; p = 0.02). CONCLUSIONS: In this study, we observed a decrease in the thymic function with age based on the fact that the level of T-cell receptor excision circles in the peripheral blood positively correlated with the proportion of regulatory T cells in healthy children and adolescents. These findings indicate that although T-cell receptor excision circles and regulatory T cells levels decrease with age, homeostasis of the immune system and relative regulatory T cells population levels are maintained in the peripheral blood.
Palavra-chave T lymphocytes
Thymus
Foxp3
T-cell Receptor
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Número do financiamento 2008/58238-4
Data de publicação 2012-01-01
Publicado em Clinics. Faculdade de Medicina / USP, v. 67, n. 5, p. 425-429, 2012.
ISSN 1807-5932 (Sherpa/Romeo, fator de impacto)
Publicador Faculdade de Medicina / USP
Extensão 425-429
Fonte http://dx.doi.org/10.6061/clinics/2012(05)04
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000304827700004
SciELO S1807-59322012000500004 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/6806

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