Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion

Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion

Autor Fernandes, Maria Cecília Google Scholar
Andrade, Leonardo Rodrigues De Google Scholar
Andrews, Norma Windsor Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Instituição University of Maryland Department of Cell Biology and Genetics
National Institutes of Health National Institute on Deafness and Other Communication Disorder Laboratory of Cell Structure and Dynamics, Section on Structural Cell Biology
Universidade Federal do Rio de Janeiro Centro de Ciências da Saúde Instituto de Ciências Biomédic
Universidade Federal de São Paulo (UNIFESP)
Resumo It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.
Palavra-chave Trypanosoma cruzi
cell invasion
cytoskeleton
membrane protrusion
Idioma Inglês
Data de publicação 2011-12-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 8, p. 1014-1016, 2011.
ISSN 0074-0276 (Sherpa/Romeo, fator de impacto)
Publicador Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 1014-1016
Fonte http://dx.doi.org/10.1590/S0074-02762011000800019
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000299068800019
SciELO S0074-02762011000800019 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/6720

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